Neuroblastoma (NB) is a common malignant solid tumor in children and accounts for 15% of childhood cancer mortality. Amplification of the N-Myc oncogene is a well-established poor prognostic marker in NB patients and strongly correlates with higher tumor aggression and resistance to treatment. New therapies for patients with N-Myc-amplified NB need to be developed. After treating NB cells with BSAO/SPM, the detection of apoptosis was determined after annexin V-FITC labeling and DNA staining with propidium iodide. The mitochondrial membrane potential activity was checked, labeling cells with the probe JC-1 dye. We analyzed, by real-time RT-PCR, the transcript of genes involved in the apoptotic process, to determine possible down- or upregulation of mRNAs after the treatment on SJNKP and the N-Myc-amplified IMR5 cell lines with BSAO/SPM. The experiments were carried out considering the proapoptotic genes Tp53 and caspase-3. After treatment with BSAO/SPM, both cell lines displayed increased mRNA levels for all these proapoptotic genes. Western blotting analysis with PARP and caspase-3 antibody support that BSAO/SPM treatment induces high levels of apoptosis in cells. The major conclusion is that BSAO/SPM treatment leads to antiproliferative and cytotoxic activity of both NB cell lines, associated with activation of apoptosis.

Enzymatic spermine metabolites induce apoptosis associated with increase of p53, caspase-3 and miR-34a in both neuroblastoma cells, SJNKP and the N-Myc-amplified form IMR5 / Kanamori, Yuta; Finotti, Alessia; Di Magno, Laura; Canettieri, Gianluca; Tahara, Tomoaki; Timeus, Fabio; Greco, Antonio; Tirassa, Paola; Gasparello, Jessica; Fino, Pasquale; Di Liegro, Carlo Maria; Proia, Patrizia; Schiera, Gabriella; Di Liegro, Italia; Gambari, Roberto; Agostinelli, Enzo. - In: CELLS. - ISSN 2073-4409. - 10:8(2021), p. 1950. [10.3390/cells10081950]

Enzymatic spermine metabolites induce apoptosis associated with increase of p53, caspase-3 and miR-34a in both neuroblastoma cells, SJNKP and the N-Myc-amplified form IMR5

Kanamori, Yuta;Di Magno, Laura;Canettieri, Gianluca;Tahara, Tomoaki;Fino, Pasquale;Agostinelli, Enzo
2021

Abstract

Neuroblastoma (NB) is a common malignant solid tumor in children and accounts for 15% of childhood cancer mortality. Amplification of the N-Myc oncogene is a well-established poor prognostic marker in NB patients and strongly correlates with higher tumor aggression and resistance to treatment. New therapies for patients with N-Myc-amplified NB need to be developed. After treating NB cells with BSAO/SPM, the detection of apoptosis was determined after annexin V-FITC labeling and DNA staining with propidium iodide. The mitochondrial membrane potential activity was checked, labeling cells with the probe JC-1 dye. We analyzed, by real-time RT-PCR, the transcript of genes involved in the apoptotic process, to determine possible down- or upregulation of mRNAs after the treatment on SJNKP and the N-Myc-amplified IMR5 cell lines with BSAO/SPM. The experiments were carried out considering the proapoptotic genes Tp53 and caspase-3. After treatment with BSAO/SPM, both cell lines displayed increased mRNA levels for all these proapoptotic genes. Western blotting analysis with PARP and caspase-3 antibody support that BSAO/SPM treatment induces high levels of apoptosis in cells. The major conclusion is that BSAO/SPM treatment leads to antiproliferative and cytotoxic activity of both NB cell lines, associated with activation of apoptosis.
2021
Polyamine; neuroblastoma; apoptosis; microRNA; mitochondria; reactive oxygen species; oncotherapy
01 Pubblicazione su rivista::01a Articolo in rivista
Enzymatic spermine metabolites induce apoptosis associated with increase of p53, caspase-3 and miR-34a in both neuroblastoma cells, SJNKP and the N-Myc-amplified form IMR5 / Kanamori, Yuta; Finotti, Alessia; Di Magno, Laura; Canettieri, Gianluca; Tahara, Tomoaki; Timeus, Fabio; Greco, Antonio; Tirassa, Paola; Gasparello, Jessica; Fino, Pasquale; Di Liegro, Carlo Maria; Proia, Patrizia; Schiera, Gabriella; Di Liegro, Italia; Gambari, Roberto; Agostinelli, Enzo. - In: CELLS. - ISSN 2073-4409. - 10:8(2021), p. 1950. [10.3390/cells10081950]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1569360
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