In unicellular organisms like yeasts, which do not have specialized tissues for protection against environmental challenges, the presence of cellular mechanisms to respond and adapt to stress conditions is fundamental. In this work, we aimed to investigate the response to environmental light in Kluyveromyces lactis. Yeast lacks specialized light-sensing proteins; however, Saccharomyces cerevisiae has been reported to respond to light by increasing hydrogen peroxide level and triggering nuclear translocation of Msn2. This is a stress-sensitive transcription factor also present in K. lactis. To investigate light response in this yeast, we analyzed the different phenotypes generated by the deletion of the hypoxia responsive and lipid biosynthesis transcription factor KlMga2. Alterations in growth rate, mitochondrial functioning, ROS metabolism, and fatty acid biosynthesis provide evidence that light was a source of stress in K. lactis and that KlMga2 had a role in the light-stress response. The involvement of KlMsn2 and KlCrz1 in light stress was also explored, but the latter showed no function in this response.

Light-stress response mediated by the transcription factor KlMga2 in the yeast Kluyveromyces lactis / Camponeschi, Ilaria; Montanari, Arianna; Beccaccioli, Marzia; Reverberi, Massimo; Mazzoni, Cristina; Bianchi, Michele M.. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 12:(2021). [10.3389/fmicb.2021.705012]

Light-stress response mediated by the transcription factor KlMga2 in the yeast Kluyveromyces lactis

Camponeschi, Ilaria;Montanari, Arianna;Beccaccioli, Marzia;Reverberi, Massimo;Mazzoni, Cristina;Bianchi, Michele M.
2021

Abstract

In unicellular organisms like yeasts, which do not have specialized tissues for protection against environmental challenges, the presence of cellular mechanisms to respond and adapt to stress conditions is fundamental. In this work, we aimed to investigate the response to environmental light in Kluyveromyces lactis. Yeast lacks specialized light-sensing proteins; however, Saccharomyces cerevisiae has been reported to respond to light by increasing hydrogen peroxide level and triggering nuclear translocation of Msn2. This is a stress-sensitive transcription factor also present in K. lactis. To investigate light response in this yeast, we analyzed the different phenotypes generated by the deletion of the hypoxia responsive and lipid biosynthesis transcription factor KlMga2. Alterations in growth rate, mitochondrial functioning, ROS metabolism, and fatty acid biosynthesis provide evidence that light was a source of stress in K. lactis and that KlMga2 had a role in the light-stress response. The involvement of KlMsn2 and KlCrz1 in light stress was also explored, but the latter showed no function in this response.
2021
desaturase; fungi; lipid; membrane; ROS
01 Pubblicazione su rivista::01a Articolo in rivista
Light-stress response mediated by the transcription factor KlMga2 in the yeast Kluyveromyces lactis / Camponeschi, Ilaria; Montanari, Arianna; Beccaccioli, Marzia; Reverberi, Massimo; Mazzoni, Cristina; Bianchi, Michele M.. - In: FRONTIERS IN MICROBIOLOGY. - ISSN 1664-302X. - 12:(2021). [10.3389/fmicb.2021.705012]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1568259
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