Mitochondrial RTG-dependent retrograde signaling, whose regulators have been char- acterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be mod- ulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of RTG signaling in salt-induced osmotic stress and its interaction with HOG1. Wild-type and mutant cells, lacking HOG1 and/or RTG genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that RTG2, the main upstream regu- lator of the RTG pathway, contributes to osmoadaptation in an HOG1-dependent manner and that, with RTG3, it is notably involved in a late phase of growth. Our data demonstrate that impairment of RTG signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that HOG1 and the RTG pathway may interact sequentially in the stress signaling cascade and that the RTG pathway may play a role in inter-organellar metabolic communication for osmoadaptation.

RTG signaling sustains mitochondrial respiratory capacity in HOG1-dependent osmoadaptation / Guaragnella, Nicoletta; Agrimi, Gennaro; Scarcia, Pasquale; Suriano, Clelia; Pisano, Isabella; Bobba, Antonella; Mazzoni, Cristina; Palmieri, Luigi; Giannattasio, Sergio. - In: MICROORGANISMS. - ISSN 2076-2607. - 9:9(2021), p. 1894. [10.3390/microorganisms9091894]

RTG signaling sustains mitochondrial respiratory capacity in HOG1-dependent osmoadaptation

Mazzoni, Cristina
Conceptualization
;
2021

Abstract

Mitochondrial RTG-dependent retrograde signaling, whose regulators have been char- acterized in Saccharomyces cerevisiae, plays a recognized role under various environmental stresses. Of special significance, the activity of the transcriptional complex Rtg1/3 has been shown to be mod- ulated by Hog1, the master regulator of the high osmolarity glycerol pathway, in response to osmotic stress. The present work focuses on the role of RTG signaling in salt-induced osmotic stress and its interaction with HOG1. Wild-type and mutant cells, lacking HOG1 and/or RTG genes, are compared with respect to cell growth features, retrograde signaling activation and mitochondrial function in the presence and in the absence of high osmostress. We show that RTG2, the main upstream regu- lator of the RTG pathway, contributes to osmoadaptation in an HOG1-dependent manner and that, with RTG3, it is notably involved in a late phase of growth. Our data demonstrate that impairment of RTG signaling causes a decrease in mitochondrial respiratory capacity exclusively under osmostress. Overall, these results suggest that HOG1 and the RTG pathway may interact sequentially in the stress signaling cascade and that the RTG pathway may play a role in inter-organellar metabolic communication for osmoadaptation.
2021
RTG signaling; HOG1; osmoadaptation; mitochondria; respiratory capacity; stress re- sponse; metabolism
01 Pubblicazione su rivista::01a Articolo in rivista
RTG signaling sustains mitochondrial respiratory capacity in HOG1-dependent osmoadaptation / Guaragnella, Nicoletta; Agrimi, Gennaro; Scarcia, Pasquale; Suriano, Clelia; Pisano, Isabella; Bobba, Antonella; Mazzoni, Cristina; Palmieri, Luigi; Giannattasio, Sergio. - In: MICROORGANISMS. - ISSN 2076-2607. - 9:9(2021), p. 1894. [10.3390/microorganisms9091894]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1568220
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