Introduction Cystic echinococcosis is a globally distributed zoonotic disease of great medical and veterinary importance, which is caused by the cestode Echinococcus granulosus sensu lato. In Ukraine, two areas of the prominent circulation of the parasite are established, the southern steppe zone with sheep as the main transmitter, and the northern forest-steppe zone and Polissia, where pigs are mainly responsible for maintaining the E. granulosus transmission. Methods Given that only a few studies have so far addressed the genetic diversity of the parasite in Ukraine, we have sequenced partial mitochondrial genes of cytochrome c oxidase 1 (789 bp), NADH dehydrogenase 1 (602 bp) and 12S rRNA (333–334 bp) in pig metacestodes from the Sumy region (farms close to Sumy, northeastern Ukraine) and the Kyiv region (a farm in Bila Tserkva, central Ukraine). Results Four isolates from four pigs in the Sumy region were identifed as E. canadensis (G7 genotype), the major E. granulosus s.l. species circulating in Eastern Europe, including the three microvariants (G7A, G7B, G7C). Three isolates from the two pigs in the Kyiv region were classifed as E. granulosus s.s. (G1 genotype), including one microvariant (G1A). Conclusion To our knowledge, this is the frst genetic record of E. granulosus s.s. with the presumed highest infectivity and virulence among the E. granulosus s.l. species in Ukraine. The fnding has implications for public health as local control programmes should take into consideration diferent development rate of this parasite in dogs and the greater risk of the species for human infection.

Molecular Study of Echinococcus granulosus Cestodes in Ukraine and the First Genetic Identification of Echinococcus granulosus Sensu Stricto (G1 Genotype) in the Country / Šnábel, Viliam; Kuzmina, Tetiana A.; Antipov, Anatoliy A.; Yemets, Oleksandr M.; Cavallero, Serena; Miterpáková, Martina; D’Amelio, Stefano; Antolová, Daniela; Vasilková, Zuzana; Sałamatin, Rusłan. - In: ACTA PARASITOLOGICA. - ISSN 1230-2821. - (2021), pp. 1-11. [10.1007/s11686-021-00450-z]

Molecular Study of Echinococcus granulosus Cestodes in Ukraine and the First Genetic Identification of Echinococcus granulosus Sensu Stricto (G1 Genotype) in the Country

Cavallero, Serena;D’Amelio, Stefano;
2021

Abstract

Introduction Cystic echinococcosis is a globally distributed zoonotic disease of great medical and veterinary importance, which is caused by the cestode Echinococcus granulosus sensu lato. In Ukraine, two areas of the prominent circulation of the parasite are established, the southern steppe zone with sheep as the main transmitter, and the northern forest-steppe zone and Polissia, where pigs are mainly responsible for maintaining the E. granulosus transmission. Methods Given that only a few studies have so far addressed the genetic diversity of the parasite in Ukraine, we have sequenced partial mitochondrial genes of cytochrome c oxidase 1 (789 bp), NADH dehydrogenase 1 (602 bp) and 12S rRNA (333–334 bp) in pig metacestodes from the Sumy region (farms close to Sumy, northeastern Ukraine) and the Kyiv region (a farm in Bila Tserkva, central Ukraine). Results Four isolates from four pigs in the Sumy region were identifed as E. canadensis (G7 genotype), the major E. granulosus s.l. species circulating in Eastern Europe, including the three microvariants (G7A, G7B, G7C). Three isolates from the two pigs in the Kyiv region were classifed as E. granulosus s.s. (G1 genotype), including one microvariant (G1A). Conclusion To our knowledge, this is the frst genetic record of E. granulosus s.s. with the presumed highest infectivity and virulence among the E. granulosus s.l. species in Ukraine. The fnding has implications for public health as local control programmes should take into consideration diferent development rate of this parasite in dogs and the greater risk of the species for human infection.
2021
echinococcus granulosus; echinococcus canadensis; ukraine; haplotype; pig
01 Pubblicazione su rivista::01a Articolo in rivista
Molecular Study of Echinococcus granulosus Cestodes in Ukraine and the First Genetic Identification of Echinococcus granulosus Sensu Stricto (G1 Genotype) in the Country / Šnábel, Viliam; Kuzmina, Tetiana A.; Antipov, Anatoliy A.; Yemets, Oleksandr M.; Cavallero, Serena; Miterpáková, Martina; D’Amelio, Stefano; Antolová, Daniela; Vasilková, Zuzana; Sałamatin, Rusłan. - In: ACTA PARASITOLOGICA. - ISSN 1230-2821. - (2021), pp. 1-11. [10.1007/s11686-021-00450-z]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1562820
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