Ras gene family members play a relevant role in cancer, especially when they are mutated. However, they may play additional roles in other conditions beside cancer. We performed gene expression analysis using the NanoString PanCancer IO 360 panel in the peripheral blood mononuclear cell (PBMC) of six COVID-19 patients and we found that H-Ras gene was significantly upregulated, while both K-Ras and N-Ras genes were downregulated. In particular, H-Ras gene upregulation was more evident in COVID-19 patients with a more severe disease. We compared our results with those obtained by analyzing two different and independent datasets, including a total of 53 COVID-19 patients, in which the gene expression analysis was performed using the Immunology_V2 panel. Comparative analysis of the H-Ras gene expression in these patients confirmed our preliminary results. In both of them, in fact, we were able to confirm the upregulation of the expression of the H-Ras gene. The exact role of this specific upregulation of the H-Ras gene in response to SARS-CoV-2 infection and its possible role in cancer still remains to be elucidated. In conclusion, H-Ras gene participates to the host immune response to SARS-CoV-2 virus infection, especially in patients affected by the most severe form of the COVID-19.
I membri della famiglia del gene Ras svolgono un ruolo rilevante nel cancro, specialmente quando sono mutati. Tuttavia, possono svolgere ruoli aggiuntivi in altre condizioni oltre al cancro. Abbiamo eseguito l'analisi dell'espressione genica utilizzando il pannello NanoString PanCancer IO 360 nelle cellule mononucleate del sangue periferico (PBMC) di sei pazienti COVID-19 e abbiamo scoperto che il gene H-Ras era significativamente sovraregolato, mentre entrambi i geni K-Ras e N-Ras erano sottoregolato. In particolare, l'upregulation del gene H-Ras era più evidente nei pazienti COVID-19 con una malattia più grave. Abbiamo confrontato i nostri risultati con quelli ottenuti analizzando due set di dati diversi e indipendenti, tra cui un totale di 53 pazienti COVID-19, in cui l'analisi dell'espressione genica è stata eseguita utilizzando il pannello Immunology_V2. L'analisi comparativa dell'espressione del gene H-Ras in questi pazienti ha confermato i nostri risultati preliminari. In entrambi, infatti, abbiamo potuto confermare la sovraregolazione dell'espressione del gene H-Ras. Il ruolo esatto di questa specifica sovraregolazione del gene H-Ras in risposta all'infezione da SARS-CoV-2 e il suo possibile ruolo nel cancro devono ancora essere chiariti. In conclusione, il gene H-Ras partecipa alla risposta immunitaria dell'ospite all'infezione da virus SARS-CoV-2, specialmente nei pazienti affetti dalla forma più grave del COVID-19.
H-Ras gene takes part to the host immune response to COVID-19 / Sciacchitano, Salvatore; Sacconi, Andrea; De Vitis, Claudia; Blandino, Giovanni; Piaggio, Giulia; Salvati, Valentina; Napoli, Christian; Marchetti, Paolo; Taurelli, Beatrice Salimbeni; Coluzzi, Flaminia; Rocco, Monica; Vecchione, Andrea; Anibaldi, Paolo; Marcolongo, Adriano; Ciliberto, Gennaro; Mancini, Rita; Capalbo, Carlo. - In: CELL DEATH DISCOVERY. - ISSN 2058-7716. - 7:1(2021), pp. 1-3. [10.1038/s41420-021-00541-w]
H-Ras gene takes part to the host immune response to COVID-19
Sciacchitano, SalvatorePrimo
Membro del Collaboration Group
;De Vitis, ClaudiaMethodology
;Napoli, ChristianSupervision
;Marchetti, PaoloSupervision
;Coluzzi, Flaminia;Rocco, Monica;Vecchione, Andrea;Anibaldi, Paolo;Mancini, RitaPenultimo
Supervision
;Capalbo, Carlo
Ultimo
2021
Abstract
Ras gene family members play a relevant role in cancer, especially when they are mutated. However, they may play additional roles in other conditions beside cancer. We performed gene expression analysis using the NanoString PanCancer IO 360 panel in the peripheral blood mononuclear cell (PBMC) of six COVID-19 patients and we found that H-Ras gene was significantly upregulated, while both K-Ras and N-Ras genes were downregulated. In particular, H-Ras gene upregulation was more evident in COVID-19 patients with a more severe disease. We compared our results with those obtained by analyzing two different and independent datasets, including a total of 53 COVID-19 patients, in which the gene expression analysis was performed using the Immunology_V2 panel. Comparative analysis of the H-Ras gene expression in these patients confirmed our preliminary results. In both of them, in fact, we were able to confirm the upregulation of the expression of the H-Ras gene. The exact role of this specific upregulation of the H-Ras gene in response to SARS-CoV-2 infection and its possible role in cancer still remains to be elucidated. In conclusion, H-Ras gene participates to the host immune response to SARS-CoV-2 virus infection, especially in patients affected by the most severe form of the COVID-19.File | Dimensione | Formato | |
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