Herein, we present a novel electrode platform for H 2 O 2 detection based on the immobilization of recombinant Tobacco Peroxidase (r-TOP) onto graphite electrodes (G) modified with p -phenylenediamine ( p -PD) diazonium cation grafted multi-walled carbon nanotubes (MWCNTs). The employment of both p -phenylenediamine moieties and covalent cross-linking by using glutaraldehyde allowed to enhance the sensitivity, stability and selectivity toward H 2 O 2 detection, as well as preventing the enzyme inactivation due to the electro-Fenton reaction. These results are mainly ascribed to a beneficial enzyme immobilization leading to a correct orientation achieved through cross-linking the enzyme in combination with interaction between the uncoupled -NH 2 groups (mainly uncharged at pH 7, considering a pK a of 4.6) available on the electrode surface and the enzyme. In particular, the electrode based on the r-TOP/ p -PD/MWCNTs/G platform showed a lower limit of detection of 1.8 µM H 2 O 2 , a extended linear range between 6 and 900 µM H 2 O 2 , as well as a significant increase in sensitivity (63.1 ± 0.1 µA mM -1 cm -2 ) compared with previous work based on TOP. The developed enzyme electrode showed a great stability, losing only 17.5% of its response along 30 days. Finally, the r-TOP/ p -PD/MWCNTs/G electrode was tested as a screening analytical method for the detection of H 2 O 2 in food samples.

Sensitive hydrogen peroxide biosensor based on Tobacco Peroxidase immobilized on p‐phenylenediamine diazonium grafted CNTs. Preventing Fenton‐like inactivation at negative potential / Ciogli, Leonardo; Zumpano, Rosaceleste; Poloznikov, Andrey A.; Hushpulian, Dmitry M.; Tishkov, Vladimir I.; Andreu, Rafael; Gorton, Lo; Mazzei, Franco; Favero, Gabriele; Bollella, Paolo. - In: CHEMELECTROCHEM. - ISSN 2196-0216. - 8:13(2021), pp. 2495-2504. [10.1002/celc.202100341]

Sensitive hydrogen peroxide biosensor based on Tobacco Peroxidase immobilized on p‐phenylenediamine diazonium grafted CNTs. Preventing Fenton‐like inactivation at negative potential

Leonardo Ciogli
Primo
;
Rosaceleste Zumpano
Secondo
;
Franco Mazzei;Gabriele Favero
Penultimo
;
2021

Abstract

Herein, we present a novel electrode platform for H 2 O 2 detection based on the immobilization of recombinant Tobacco Peroxidase (r-TOP) onto graphite electrodes (G) modified with p -phenylenediamine ( p -PD) diazonium cation grafted multi-walled carbon nanotubes (MWCNTs). The employment of both p -phenylenediamine moieties and covalent cross-linking by using glutaraldehyde allowed to enhance the sensitivity, stability and selectivity toward H 2 O 2 detection, as well as preventing the enzyme inactivation due to the electro-Fenton reaction. These results are mainly ascribed to a beneficial enzyme immobilization leading to a correct orientation achieved through cross-linking the enzyme in combination with interaction between the uncoupled -NH 2 groups (mainly uncharged at pH 7, considering a pK a of 4.6) available on the electrode surface and the enzyme. In particular, the electrode based on the r-TOP/ p -PD/MWCNTs/G platform showed a lower limit of detection of 1.8 µM H 2 O 2 , a extended linear range between 6 and 900 µM H 2 O 2 , as well as a significant increase in sensitivity (63.1 ± 0.1 µA mM -1 cm -2 ) compared with previous work based on TOP. The developed enzyme electrode showed a great stability, losing only 17.5% of its response along 30 days. Finally, the r-TOP/ p -PD/MWCNTs/G electrode was tested as a screening analytical method for the detection of H 2 O 2 in food samples.
2021
recombinant Tobacco Peroxidase; p-phenylenediamine diazonium cations; electro-Fenton reaction; hydrogen peroxide biosensor; modified electrodes
01 Pubblicazione su rivista::01a Articolo in rivista
Sensitive hydrogen peroxide biosensor based on Tobacco Peroxidase immobilized on p‐phenylenediamine diazonium grafted CNTs. Preventing Fenton‐like inactivation at negative potential / Ciogli, Leonardo; Zumpano, Rosaceleste; Poloznikov, Andrey A.; Hushpulian, Dmitry M.; Tishkov, Vladimir I.; Andreu, Rafael; Gorton, Lo; Mazzei, Franco; Favero, Gabriele; Bollella, Paolo. - In: CHEMELECTROCHEM. - ISSN 2196-0216. - 8:13(2021), pp. 2495-2504. [10.1002/celc.202100341]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1558503
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