A silica-chitosan support was employed for laccase immobilization. The hybrid support was obtained using calcium ion as linking agent that coordinates silanol and hydroxyl groups of chitosan. The insoluble biocatalyst was then packed in a column and used in a flow system for phenol removal. The immobilized enzyme reactor (IMER) showed a good storage stability (70 % of activity in 70 days) and good reusability (90−50 % of catalytic activity at the 4th reuse in function of chitosan type). The best performance for the phenol removal was obtained with a low molecular weight chitosan from crab shells at pH 5 and with a flow rate of 0.7 mL/min. The apparent Michaelis–Menten (Vmaxapp, Kmapp) and the inherent (Vmaxinh, Kminh) constants were also determined to evaluate the influence of the phenol structure on the performance of the system. The enzymatic oxidation of a phenol mixture (4-methylcatechol, catechol, caffeic acid, syringic acid, vanillic acid, p-coumaric acid, and tyrosol) was followed for 21 h in a continuous mode by HPLC. The phenol mixture removal of 90 % was also confirmed by Folin‐Ciocalteu assay.

Design of bioreactor based on immobilized laccase on silica-chitosan support for phenol removal in continuous mode / Girelli, A. M.; Quattrocchi, L.; Scuto, F. R.. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - 337:(2021), pp. 8-17. [10.1016/j.jbiotec.2021.06.010]

Design of bioreactor based on immobilized laccase on silica-chitosan support for phenol removal in continuous mode

Girelli, A. M.
;
Quattrocchi, L.;Scuto, F. R.
2021

Abstract

A silica-chitosan support was employed for laccase immobilization. The hybrid support was obtained using calcium ion as linking agent that coordinates silanol and hydroxyl groups of chitosan. The insoluble biocatalyst was then packed in a column and used in a flow system for phenol removal. The immobilized enzyme reactor (IMER) showed a good storage stability (70 % of activity in 70 days) and good reusability (90−50 % of catalytic activity at the 4th reuse in function of chitosan type). The best performance for the phenol removal was obtained with a low molecular weight chitosan from crab shells at pH 5 and with a flow rate of 0.7 mL/min. The apparent Michaelis–Menten (Vmaxapp, Kmapp) and the inherent (Vmaxinh, Kminh) constants were also determined to evaluate the influence of the phenol structure on the performance of the system. The enzymatic oxidation of a phenol mixture (4-methylcatechol, catechol, caffeic acid, syringic acid, vanillic acid, p-coumaric acid, and tyrosol) was followed for 21 h in a continuous mode by HPLC. The phenol mixture removal of 90 % was also confirmed by Folin‐Ciocalteu assay.
2021
immobilized laccase; continuous mode; silica-chitosan support
01 Pubblicazione su rivista::01a Articolo in rivista
Design of bioreactor based on immobilized laccase on silica-chitosan support for phenol removal in continuous mode / Girelli, A. M.; Quattrocchi, L.; Scuto, F. R.. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - 337:(2021), pp. 8-17. [10.1016/j.jbiotec.2021.06.010]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1557578
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