The visualization of heterogeneous morphology, segmentation and quantification of image features is a crucial point for nonlinear optics microscopy applications, spanning from imaging of living cells or tissues to biomedical diagnostic. In this paper, a methodology combining stimulated Raman scattering microscopy and image analysis technique is presented. The basic idea is to join the potential of vibrational contrast of stimulated Raman scattering and the strength of imaging analysis technique in order to delineate subcellular morphology with chemical specificity. Validation tests on label free imaging of polystyrene-beads and of adipocyte cells are reported and discussed.

Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques / D'Arco, A.; Brancati, N.; Ferrara, M. A.; Indolfi, M.; Frucci, M.; Sirleto, L.. - In: BIOMEDICAL OPTICS EXPRESS. - ISSN 2156-7085. - 7:5(2016), pp. 1853-1864. [10.1364/BOE.7.001853]

Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques

D'arco A.
Primo
;
2016

Abstract

The visualization of heterogeneous morphology, segmentation and quantification of image features is a crucial point for nonlinear optics microscopy applications, spanning from imaging of living cells or tissues to biomedical diagnostic. In this paper, a methodology combining stimulated Raman scattering microscopy and image analysis technique is presented. The basic idea is to join the potential of vibrational contrast of stimulated Raman scattering and the strength of imaging analysis technique in order to delineate subcellular morphology with chemical specificity. Validation tests on label free imaging of polystyrene-beads and of adipocyte cells are reported and discussed.
2016
Scattering stimulated; Ultrafast nonlinear optics; Nonlinear microscopy; Imaging systems; Image processing
01 Pubblicazione su rivista::01a Articolo in rivista
Subcellular chemical and morphological analysis by stimulated Raman scattering microscopy and image analysis techniques / D'Arco, A.; Brancati, N.; Ferrara, M. A.; Indolfi, M.; Frucci, M.; Sirleto, L.. - In: BIOMEDICAL OPTICS EXPRESS. - ISSN 2156-7085. - 7:5(2016), pp. 1853-1864. [10.1364/BOE.7.001853]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1553327
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