In this study, a new scaffold fabrication method based on the combination of a series of stabilization processes was set up to obtain chitosan scaffolds with improved mechanical properties for regeneration of load-bearing tissues. Specifically, thermally induced phase separation (TIPS) of chitosan solutions was used to obtain an open structure which was then stabilized by freeze-gelation and photo cross-linking. Freeze-gelation combined with freeze-drying permitted to obtain a porous structure with a 95 μm-mean pore size suitable for osteoblast cells' housing. Photo-crosslinking improved by ca. three times the scaffold compressive modulus, passing from 0,8 MPa of the uncrosslinked scaffolds to 2,2 MPa of the crosslinked one. Hydrated crosslinked scaffolds showed a good elastic response, with an 80% elastic recovery for at least 5 consecutive compressive cycles. The herein reported method has the advantage to not require the use of potentially toxic cross-linking agents and may be extended to other soft materials.

Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying / Silvestro, I.; Sergi, R.; Scotto d'Abusco, A.; Mariano, A.; Martinelli, A.; Piozzi, A.; Francolini, I.. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - 267:(2021), p. 118156. [10.1016/j.carbpol.2021.118156]

Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying

Silvestro I.;Sergi R.;Scotto d'Abusco A.;Mariano A.;Martinelli A.;Piozzi A.;Francolini I.
2021

Abstract

In this study, a new scaffold fabrication method based on the combination of a series of stabilization processes was set up to obtain chitosan scaffolds with improved mechanical properties for regeneration of load-bearing tissues. Specifically, thermally induced phase separation (TIPS) of chitosan solutions was used to obtain an open structure which was then stabilized by freeze-gelation and photo cross-linking. Freeze-gelation combined with freeze-drying permitted to obtain a porous structure with a 95 μm-mean pore size suitable for osteoblast cells' housing. Photo-crosslinking improved by ca. three times the scaffold compressive modulus, passing from 0,8 MPa of the uncrosslinked scaffolds to 2,2 MPa of the crosslinked one. Hydrated crosslinked scaffolds showed a good elastic response, with an 80% elastic recovery for at least 5 consecutive compressive cycles. The herein reported method has the advantage to not require the use of potentially toxic cross-linking agents and may be extended to other soft materials.
2021
Chitosan; fabrication method; mechanical properties; scaffolds; tissue engineering
01 Pubblicazione su rivista::01a Articolo in rivista
Chitosan scaffolds with enhanced mechanical strength and elastic response by combination of freeze gelation, photo-crosslinking and freeze-drying / Silvestro, I.; Sergi, R.; Scotto d'Abusco, A.; Mariano, A.; Martinelli, A.; Piozzi, A.; Francolini, I.. - In: CARBOHYDRATE POLYMERS. - ISSN 0144-8617. - 267:(2021), p. 118156. [10.1016/j.carbpol.2021.118156]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1549443
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