In the last decades, DNA has been considered far more than the system carrying the essential genetic instructions. Indeed, because of the remarkable properties of the base-pairing specificity and thermoreversibility of the interactions, DNA plays a central role in the design of innovative architectures at the nanoscale. Here, combining complementary DNA strands with a custom-made solution of silver nanoparticles, we realize plasmonic aggregates to exploit the sensitivity of Surface Enhanced Raman Spectroscopy (SERS) for the identification/detection of the distinctive features of DNA hybridization, both in solution and on dried samples. Moreover, SERS allows monitoring the DNA aggregation process by following the temperature variation of a specific spectroscopic marker associated with the Watson-Crick hydrogen bond formation. This temperature-dependent behavior enables us to precisely reconstruct the melting profile of the selected DNA sequences by spectroscopic measurements only.

Exploiting SERS sensitivity to monitor DNA aggregation properties / Caprara, D.; Ripanti, F.; Capocefalo, A.; Ceccarini, M.; Petrillo, C.; Postorino, P.. - In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - ISSN 0141-8130. - 170:(2021), pp. 88-93. [10.1016/j.ijbiomac.2020.12.039]

Exploiting SERS sensitivity to monitor DNA aggregation properties

Caprara D.;Ripanti F.
;
Capocefalo A.;Postorino P.
2021

Abstract

In the last decades, DNA has been considered far more than the system carrying the essential genetic instructions. Indeed, because of the remarkable properties of the base-pairing specificity and thermoreversibility of the interactions, DNA plays a central role in the design of innovative architectures at the nanoscale. Here, combining complementary DNA strands with a custom-made solution of silver nanoparticles, we realize plasmonic aggregates to exploit the sensitivity of Surface Enhanced Raman Spectroscopy (SERS) for the identification/detection of the distinctive features of DNA hybridization, both in solution and on dried samples. Moreover, SERS allows monitoring the DNA aggregation process by following the temperature variation of a specific spectroscopic marker associated with the Watson-Crick hydrogen bond formation. This temperature-dependent behavior enables us to precisely reconstruct the melting profile of the selected DNA sequences by spectroscopic measurements only.
DNA self-assembly; Melting profile; SERS spectroscopy; Silver nanoparticles; Base Pairing; DNA, Single-Stranded; Desiccation; Hydrogen Bonding; Hydroxylamine; Metal Nanoparticles; Nucleic Acid Denaturation; Silver; Solutions; Spectrum Analysis, Raman; Temperature; Nucleic Acid Hybridization
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Exploiting SERS sensitivity to monitor DNA aggregation properties / Caprara, D.; Ripanti, F.; Capocefalo, A.; Ceccarini, M.; Petrillo, C.; Postorino, P.. - In: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. - ISSN 0141-8130. - 170:(2021), pp. 88-93. [10.1016/j.ijbiomac.2020.12.039]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1548884
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