Abstract: 30-50 cyclic nucleotide phosphodiesterases (PDEs) are a large family of enzymes playing a fundamental role in the control of intracellular levels of cAMP and cGMP. Emerging evidence suggested an important role of phosphodiesterases in heart formation, but little is known about the expression of phosphodiesterases during cardiac development. In the present study, the pattern of expression and enzymatic activity of phosphodiesterases was investigated at different stages of heart formation. C57BL/6 mice were mated and embryos were collected from 14.5 to 18.5 days of development. Data obtained by qRT-PCR and Western blot analysis showed that seven different isoforms are expressed during heart development, and PDE1C, PDE2A, PDE4D, PDE5A and PDE8A are modulated from E14.5 to E18.5. In heart homogenates, the total cAMP and cGMP hydrolytic activity is constant at the evaluated times, and PDE4 accounts for the majority of the cAMP hydrolyzing ability and PDE2A accounts for cGMP hydrolysis. This study showed that a subset of PDEs is expressed in developing mice heart and some of them are modulated to maintain constant nucleotide phosphodiesterase activity in embryonic and fetal heart.

Phosphodiesterases expression during murine cardiac development / SILVA CARVALHO, THAYS MARIA; Cardarelli, Silvia; Giorgi, Mauro; Lenzi, Andrea; Isidori, Andrea M.; Naro, Fabio. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 22:5(2021), pp. 1-12. [10.3390/ijms22052593]

Phosphodiesterases expression during murine cardiac development

Thays Maria da Conceição Silva Carvalho
Membro del Collaboration Group
;
Silvia Cardarelli
Membro del Collaboration Group
;
Mauro Giorgi
Membro del Collaboration Group
;
Andrea Lenzi
Membro del Collaboration Group
;
Andrea M. Isidori
Membro del Collaboration Group
;
Fabio Naro
Membro del Collaboration Group
2021

Abstract

Abstract: 30-50 cyclic nucleotide phosphodiesterases (PDEs) are a large family of enzymes playing a fundamental role in the control of intracellular levels of cAMP and cGMP. Emerging evidence suggested an important role of phosphodiesterases in heart formation, but little is known about the expression of phosphodiesterases during cardiac development. In the present study, the pattern of expression and enzymatic activity of phosphodiesterases was investigated at different stages of heart formation. C57BL/6 mice were mated and embryos were collected from 14.5 to 18.5 days of development. Data obtained by qRT-PCR and Western blot analysis showed that seven different isoforms are expressed during heart development, and PDE1C, PDE2A, PDE4D, PDE5A and PDE8A are modulated from E14.5 to E18.5. In heart homogenates, the total cAMP and cGMP hydrolytic activity is constant at the evaluated times, and PDE4 accounts for the majority of the cAMP hydrolyzing ability and PDE2A accounts for cGMP hydrolysis. This study showed that a subset of PDEs is expressed in developing mice heart and some of them are modulated to maintain constant nucleotide phosphodiesterase activity in embryonic and fetal heart.
2021
cyclic nucleotides; heart development; phosphodiesterases
01 Pubblicazione su rivista::01a Articolo in rivista
Phosphodiesterases expression during murine cardiac development / SILVA CARVALHO, THAYS MARIA; Cardarelli, Silvia; Giorgi, Mauro; Lenzi, Andrea; Isidori, Andrea M.; Naro, Fabio. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1661-6596. - 22:5(2021), pp. 1-12. [10.3390/ijms22052593]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1540870
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