In this paper, the first phytochemical analysis of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., -linolenic acid (1), tri-linoleoyl-sn-glycerol (2), pheophorbide a ethyl ester (3), pilloin (4), sinensetin (5), yuanhuanin (6), rutamontine (7), syringin (8), p-coumaric acid (9), p-anisic acid (10) and caffeic acid (11). To the best of our knowledge, compounds (1-4, 7-8 and 10) were isolated from D. sericea for the first time during this work, whereas sinensetin (5) represents a newly identified component of the entire Thymelaeaceae family. The extract was found to possess radical scavenging against both DPPH and 2,20-azino-bis(3-thylbenzothiazoline-6-sulfonic acid (ABTS.+) radicals, with at least a 40-fold higher potency against the latter. Moreover, chelating abilities against both ferrous and ferric ions have been highlighted, thus suggesting a possible indirect antioxidant power of the extract. Although the precise bioactive compounds remain to be discovered, the polyphenolic constituents, including phenolic acids, tannins and flavonoids, seem to contribute to the antioxidant power of the phytocomplex. In addition, the extract produced cytotoxic effects in MDA-MB-231 and U87-MG cancer cell lines, especially at the concentration of 625 g/mL and after 48–72 h. Further studies are required to clarify the contribution of the identified compounds in the bioactivities of the extract and to support possible future applications.

Phytochemical analysis and biological activities of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Central Italy / Frezza, Claudio; Venditti, Alessandro; DE VITA, Daniela; Sciubba, Fabio; Tomai, Pierpaolo; Franceschin, Marco; Di Cecco, Mirella; Ciaschetti, Giampiero; DI SOTTO, Antonella; Stringaro, Annarita; Colone, Marisa; Gentili, Alessandra; Serafini, Mauro; Bianco, Armandodoriano. - In: BIOMOLECULES. - ISSN 2218-273X. - 11:3(2021), pp. 1-17. [10.3390/biom11030379]

Phytochemical analysis and biological activities of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Central Italy

Claudio Frezza
;
Daniela De Vita;Fabio Sciubba;Pierpaolo Tomai;Antonella Di Sotto;Alessandra Gentili;Mauro Serafini
;
Armandodoriano Bianco
2021

Abstract

In this paper, the first phytochemical analysis of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Italy and its biological activities were reported. Eleven compounds were identified i.e., -linolenic acid (1), tri-linoleoyl-sn-glycerol (2), pheophorbide a ethyl ester (3), pilloin (4), sinensetin (5), yuanhuanin (6), rutamontine (7), syringin (8), p-coumaric acid (9), p-anisic acid (10) and caffeic acid (11). To the best of our knowledge, compounds (1-4, 7-8 and 10) were isolated from D. sericea for the first time during this work, whereas sinensetin (5) represents a newly identified component of the entire Thymelaeaceae family. The extract was found to possess radical scavenging against both DPPH and 2,20-azino-bis(3-thylbenzothiazoline-6-sulfonic acid (ABTS.+) radicals, with at least a 40-fold higher potency against the latter. Moreover, chelating abilities against both ferrous and ferric ions have been highlighted, thus suggesting a possible indirect antioxidant power of the extract. Although the precise bioactive compounds remain to be discovered, the polyphenolic constituents, including phenolic acids, tannins and flavonoids, seem to contribute to the antioxidant power of the phytocomplex. In addition, the extract produced cytotoxic effects in MDA-MB-231 and U87-MG cancer cell lines, especially at the concentration of 625 g/mL and after 48–72 h. Further studies are required to clarify the contribution of the identified compounds in the bioactivities of the extract and to support possible future applications.
2021
Daphne sericea Vahl; thymelaeaceae; phytochemical analysis; chemotaxonomy; polyphenols; antioxidant activities; cancer cell cytotoxicity
01 Pubblicazione su rivista::01a Articolo in rivista
Phytochemical analysis and biological activities of the ethanolic extract of Daphne sericea Vahl flowering aerial parts collected in Central Italy / Frezza, Claudio; Venditti, Alessandro; DE VITA, Daniela; Sciubba, Fabio; Tomai, Pierpaolo; Franceschin, Marco; Di Cecco, Mirella; Ciaschetti, Giampiero; DI SOTTO, Antonella; Stringaro, Annarita; Colone, Marisa; Gentili, Alessandra; Serafini, Mauro; Bianco, Armandodoriano. - In: BIOMOLECULES. - ISSN 2218-273X. - 11:3(2021), pp. 1-17. [10.3390/biom11030379]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1525063
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