Alterations of RNA homeostasis can lead to severe pathological conditions. The Survival of Motor Neuron (SMN) protein, which is reduced in Spinal Muscular Atrophy, impacts critical aspects of the RNA life cycle, such as splicing, trafficking, and translation. Increasing evidence points to a potential role of SMN in ribosome biogenesis. Our previous study revealed that SMN promotes membrane-bound ribosomal proteins (RPs), sustaining activity-dependent local translation. Here, we suggest that plasma membrane domains could be a docking site not only for RPs but also for their encoding transcripts. We have shown that SMN knockdown perturbs subcellular localization as well as translation efficiency of RPS6 mRNA. We have also shown that plasma membrane-enriched fractions from human fibroblasts retain RPS6 transcripts in an SMN-dependent manner. Furthermore, we revealed that SMN traffics with RPS6 mRNA promoting its association with caveolin-1, a key component of membrane dynamics. Overall, these findings further support the SMN-mediated crosstalk between plasma membrane dynamics and translation machinery. Importantly, our study points to a potential role of SMN in the ribosome assembly pathway by selective RPs synthesis/localization in both space and time.

SMN protein promotes membrane compartmentalization of ribosomal protein S6 transcript in human fibroblasts / Gabanella, F.; Onori, A.; Ralli, M.; Greco, A.; Passananti, C.; Di Certo, M. G.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 10:1(2020). [10.1038/s41598-020-76174-3]

SMN protein promotes membrane compartmentalization of ribosomal protein S6 transcript in human fibroblasts

Ralli M.;Greco A.;
2020

Abstract

Alterations of RNA homeostasis can lead to severe pathological conditions. The Survival of Motor Neuron (SMN) protein, which is reduced in Spinal Muscular Atrophy, impacts critical aspects of the RNA life cycle, such as splicing, trafficking, and translation. Increasing evidence points to a potential role of SMN in ribosome biogenesis. Our previous study revealed that SMN promotes membrane-bound ribosomal proteins (RPs), sustaining activity-dependent local translation. Here, we suggest that plasma membrane domains could be a docking site not only for RPs but also for their encoding transcripts. We have shown that SMN knockdown perturbs subcellular localization as well as translation efficiency of RPS6 mRNA. We have also shown that plasma membrane-enriched fractions from human fibroblasts retain RPS6 transcripts in an SMN-dependent manner. Furthermore, we revealed that SMN traffics with RPS6 mRNA promoting its association with caveolin-1, a key component of membrane dynamics. Overall, these findings further support the SMN-mediated crosstalk between plasma membrane dynamics and translation machinery. Importantly, our study points to a potential role of SMN in the ribosome assembly pathway by selective RPs synthesis/localization in both space and time.
2020
Cell Membrane; Fibroblasts; Humans; Protein Biosynthesis; Protein Transport; RNA, Messenger; Ribosomal Protein S6; Ribosomes; Survival of Motor Neuron 1 Protein; Cell Compartmentation
01 Pubblicazione su rivista::01a Articolo in rivista
SMN protein promotes membrane compartmentalization of ribosomal protein S6 transcript in human fibroblasts / Gabanella, F.; Onori, A.; Ralli, M.; Greco, A.; Passananti, C.; Di Certo, M. G.. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 10:1(2020). [10.1038/s41598-020-76174-3]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1524864
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