Aim: Our aim was to evaluate gene expression profiling of fibroblasts from human alveolar mucosa (M), buccal attached gingiva (G) and palatal (P) tissues during early wound healing, correlating it with clinical response. Materials and Methods: M, G and P biopsies were harvested from six patients at baseline and 24 hrs after surgery. Clinical response was evaluated through Early Wound Healing Score (EHS). Fibrotic markers expression and autophagy were assessed on fibroblasts isolated from those tissues by Western blot and qRT‐PCR. Fibroblasts from two patients were subjected to RT2 profiler array, followed by network analysis of the differentially expressed genes. The expression of key genes was validated with qRT‐PCR on all patients. Results: At 24 hrs after surgery, EHS was higher in P and G than in M. In line with our clinical results, no autophagy and myofibroblast differentiation were observed in G and P. We observed significant variations in mRNA expression of key genes: RAC1, SERPINE1 and TIMP1, involved in scar formation; CDH1, ITGA4 and ITGB5, contributing to myofibroblasts differentiation; IL6 and CXCL1, involved in inflammation. Conclusions: We identified some genes involved in periodontal soft tissues clinical outcome, providing novel insights into the molecular mechanisms of oral repair (ClinicalTrial.gov‐NCT04202822).

Gene expression profiles of oral soft tissue-derived fibroblast from healing wounds: correlation with clinical outcome, autophagy activation and fibrotic markers expression / Rojas, Mariana Andrea; Ceccarelli, Simona; Gerini, Giulia; Vescarelli, Enrica; Marini, Lorenzo; Marchese, Cinzia; Pilloni, Andrea. - In: JOURNAL OF CLINICAL PERIODONTOLOGY. - ISSN 0303-6979. - 48:5(2021), pp. 705-720. [10.1111/jcpe.13439]

Gene expression profiles of oral soft tissue-derived fibroblast from healing wounds: correlation with clinical outcome, autophagy activation and fibrotic markers expression

Rojas, Mariana Andrea
Co-primo
;
Ceccarelli, Simona
Co-primo
;
Gerini, Giulia;Vescarelli, Enrica;Marini, Lorenzo;Marchese, Cinzia
Penultimo
;
Pilloni, Andrea
Ultimo
2021

Abstract

Aim: Our aim was to evaluate gene expression profiling of fibroblasts from human alveolar mucosa (M), buccal attached gingiva (G) and palatal (P) tissues during early wound healing, correlating it with clinical response. Materials and Methods: M, G and P biopsies were harvested from six patients at baseline and 24 hrs after surgery. Clinical response was evaluated through Early Wound Healing Score (EHS). Fibrotic markers expression and autophagy were assessed on fibroblasts isolated from those tissues by Western blot and qRT‐PCR. Fibroblasts from two patients were subjected to RT2 profiler array, followed by network analysis of the differentially expressed genes. The expression of key genes was validated with qRT‐PCR on all patients. Results: At 24 hrs after surgery, EHS was higher in P and G than in M. In line with our clinical results, no autophagy and myofibroblast differentiation were observed in G and P. We observed significant variations in mRNA expression of key genes: RAC1, SERPINE1 and TIMP1, involved in scar formation; CDH1, ITGA4 and ITGB5, contributing to myofibroblasts differentiation; IL6 and CXCL1, involved in inflammation. Conclusions: We identified some genes involved in periodontal soft tissues clinical outcome, providing novel insights into the molecular mechanisms of oral repair (ClinicalTrial.gov‐NCT04202822).
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/1488788
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