Polymerase chain reaction (PCR)-based genotyping of mutations in the voltage-sensitive sodium channel (vssc) associated with resistance to pyrethroid insecticides is widely used and represents a potential early warning and monitoring system for insecticide resistance arising in mosquito populations, which are vectors of different human pathogens. In the secondary vector Aedes albopictus-an Asian species that has invaded and colonized the whole world, including temperate regions-sequencing of domain II of the vssc gene is still needed to detect the V1016G mutation associated with pyrethroid resistance. In this study we developed and tested a novel allele-specific PCR (AS-PCR) assay to genotype the V1016G mutation in this species and applied it to the analysis of wild populations from Italy. The results confirm the high accuracy of the novel AS-PCR and highlight frequencies of the V1016G allele as >5% in most sampling sites, with peaks of 20-45% in coastal touristic sites where pyrethroid treatments are extensively implemented, mostly for mosquito nuisance reduction. The high frequency of this mutation observed in Italian Ae. albopictus populations should serve as a warning bell, advocating for increased monitoring and management of a phenomenon which risks neutralizing the only weapon today available to counteract (risks of) arbovirus outbreaks.

A novel allele specific polymerase chain reaction (AS-PCR) assay to detect the V1016G knockdown resistance mutation confirms Its widespread presence in aedes albopictus populations from Italy / Pichler, Verena; Mancini, Emiliano; Micocci, Martina; Calzetta, Maria; Arnoldi, Daniele; Rizzoli, Annapaola; Lencioni, Valeria; Paoli, Francesca; Bellini, Romeo; Veronesi, Rodolfo; Martini, Simone; Drago, Andrea; De Liberato, Claudio; Ermenegildi, Arianna; Pinto, Joao; Della Torre, Alessandra; Caputo, Beniamino. - In: INSECTS. - ISSN 2075-4450. - 12:1(2021), pp. 1-12. [10.3390/insects12010079]

A novel allele specific polymerase chain reaction (AS-PCR) assay to detect the V1016G knockdown resistance mutation confirms Its widespread presence in aedes albopictus populations from Italy

Pichler, Verena;Mancini, Emiliano
Secondo
Methodology
;
Micocci, Martina;Calzetta, Maria;Bellini, Romeo;Martini, Simone;Della Torre, Alessandra
;
Caputo, Beniamino
2021

Abstract

Polymerase chain reaction (PCR)-based genotyping of mutations in the voltage-sensitive sodium channel (vssc) associated with resistance to pyrethroid insecticides is widely used and represents a potential early warning and monitoring system for insecticide resistance arising in mosquito populations, which are vectors of different human pathogens. In the secondary vector Aedes albopictus-an Asian species that has invaded and colonized the whole world, including temperate regions-sequencing of domain II of the vssc gene is still needed to detect the V1016G mutation associated with pyrethroid resistance. In this study we developed and tested a novel allele-specific PCR (AS-PCR) assay to genotype the V1016G mutation in this species and applied it to the analysis of wild populations from Italy. The results confirm the high accuracy of the novel AS-PCR and highlight frequencies of the V1016G allele as >5% in most sampling sites, with peaks of 20-45% in coastal touristic sites where pyrethroid treatments are extensively implemented, mostly for mosquito nuisance reduction. The high frequency of this mutation observed in Italian Ae. albopictus populations should serve as a warning bell, advocating for increased monitoring and management of a phenomenon which risks neutralizing the only weapon today available to counteract (risks of) arbovirus outbreaks.
2021
ades albopictus; insecticide resistance; integrated vector management; kdr genotyping; pyrethroid resistance; vector control
01 Pubblicazione su rivista::01a Articolo in rivista
A novel allele specific polymerase chain reaction (AS-PCR) assay to detect the V1016G knockdown resistance mutation confirms Its widespread presence in aedes albopictus populations from Italy / Pichler, Verena; Mancini, Emiliano; Micocci, Martina; Calzetta, Maria; Arnoldi, Daniele; Rizzoli, Annapaola; Lencioni, Valeria; Paoli, Francesca; Bellini, Romeo; Veronesi, Rodolfo; Martini, Simone; Drago, Andrea; De Liberato, Claudio; Ermenegildi, Arianna; Pinto, Joao; Della Torre, Alessandra; Caputo, Beniamino. - In: INSECTS. - ISSN 2075-4450. - 12:1(2021), pp. 1-12. [10.3390/insects12010079]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1486040
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