In this paper we describe a simple method to improve the plating efficiency in plant cell cultures. Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony trapped in the agar network. In the second stage the colonies are plated at different densities in 1% agarized medium. These colonies are self-sufficient and able to improve the cell growth by conditioning the medium.
High plating efficiency with plant cell cultures / Bellincampi, Daniela; Baduri, N; Morpurgo, G.. - In: PLANT CELL REPORTS. - ISSN 0721-7714. - STAMPA. - 4:(1985), pp. 155-157. [10.1007/BF00571305]
High plating efficiency with plant cell cultures.
BELLINCAMPI, Daniela;
1985
Abstract
In this paper we describe a simple method to improve the plating efficiency in plant cell cultures. Two-stage plating is used; in the first stage the cells are inoculated at high density in 0.2% agarized culture medium for ten days to facilitate growth; under this condition, each cell produces a single micro-colony trapped in the agar network. In the second stage the colonies are plated at different densities in 1% agarized medium. These colonies are self-sufficient and able to improve the cell growth by conditioning the medium.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
