UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention. We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2). CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements. We name “Parodi limit” the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site. Based on the MD simulations, we estimate the Parodi limit as around 70–80 Å. Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo. Our data support a “one-size-fits-all adjustable spanner” UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.

Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose:glycoprotein glucosyltransferase / Modenutti, C. P.; Blanco Capurro, J. I.; Ibba, R.; Alonzi, D. S.; Song, M. N.; Vasiljevic, S.; Kumar, A.; Chandran, A. V.; Tax, G.; Marti, L.; Hill, J. C.; Lia, A.; Hensen, M.; Waksman, T.; Rushton, J.; Rubichi, S.; Santino, A.; Marti, M. A.; Zitzmann, N.; Roversi, P.. - In: STRUCTURE. - ISSN 0969-2126. - (2021). [10.1016/j.str.2020.11.017]

Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose:glycoprotein glucosyltransferase

Kumar A.;Marti L.;
2021

Abstract

UDP-glucose:glycoprotein glucosyltransferase (UGGT) flags misfolded glycoproteins for ER retention. We report crystal structures of full-length Chaetomium thermophilum UGGT (CtUGGT), two CtUGGT double-cysteine mutants, and its TRXL2 domain truncation (CtUGGT-ΔTRXL2). CtUGGT molecular dynamics (MD) simulations capture extended conformations and reveal clamping, bending, and twisting inter-domain movements. We name “Parodi limit” the maximum distance on the same glycoprotein between a site of misfolding and an N-linked glycan that can be reglucosylated by monomeric UGGT in vitro, in response to recognition of misfold at that site. Based on the MD simulations, we estimate the Parodi limit as around 70–80 Å. Frequency distributions of distances between glycoprotein residues and their closest N-linked glycosylation sites in glycoprotein crystal structures suggests relevance of the Parodi limit to UGGT activity in vivo. Our data support a “one-size-fits-all adjustable spanner” UGGT substrate recognition model, with an essential role for the UGGT TRXL2 domain.
2021
glycoprotein folding; GT24 domain; misfold sensing; misfolding; molecular dynamics; negative-stain EM; Parodi limit; re-glucosylation; UGGT; X-ray diffraction
01 Pubblicazione su rivista::01a Articolo in rivista
Clamping, bending, and twisting inter-domain motions in the misfold-recognizing portion of UDP-glucose:glycoprotein glucosyltransferase / Modenutti, C. P.; Blanco Capurro, J. I.; Ibba, R.; Alonzi, D. S.; Song, M. N.; Vasiljevic, S.; Kumar, A.; Chandran, A. V.; Tax, G.; Marti, L.; Hill, J. C.; Lia, A.; Hensen, M.; Waksman, T.; Rushton, J.; Rubichi, S.; Santino, A.; Marti, M. A.; Zitzmann, N.; Roversi, P.. - In: STRUCTURE. - ISSN 0969-2126. - (2021). [10.1016/j.str.2020.11.017]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1482350
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 4
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 69
social impact