Objective: Effector phase of acute graft-versus-host disease (a-GVHD) is mainly mediated by donor-derived, anti-host cytotoxic T cells. T-cell homing into gut-associated lymphoid tissues is ascribed to the α4β7 integrin. We reasoned that development of intestinal a-GVHD might be triggered by recruitment in the intestinal mucosa of circulating, alloreactive, α4β7+ donor T cells. Therefore, we evaluated the correlation existing between circulating β7+ T-lymphocyte subsets early after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and occurrence of a-GVHD. Patients and Methods: Surface expression of β7 integrin on T cells was evaluated by means of direct immunofluorescence, in three-color analysis. Sixty-five patients given allo-HSCT were evaluated: 13 of them experienced intestinal a-GVHD, 14 developed a-GVHD without intestinal involvement, and 38 did not develop a-GVHD. Patients were studied early after initial signs of hematologic reconstitution and before occurrence of a-GVHD. Results: We found a significantly higher absolute number of CD8+ and a significantly lower percentage of CD8+CD45RA+β7+ T cells in patients with intestinal a-GVHD than in patients with a-GVHD without intestinal involvement (p = 0.003 and p = 0.003, respectively) or not experiencing a-GVHD (p = 0.02 and p = 0.002, respectively). In particular, we found that intestinal a-GVHD occurred in over 70% of patients showing an absolute number of CD8+ T cells ≥ 60 × 106/L and a percentage of circulating CD8+CD45RA+β7+ T cells < 35%. Conclusion: Measuring the absolute number of CD8+ T cells and percentage of CD8+CD45RA+β7+ T cells at time of hematologic reconstitution may help identify patients at risk of developing intestinal a-GVHD who could benefit from strategies aimed at hampering alloreactive T-cell homing to intestinal mucosa. © 2006 International Society for Experimental Hematology.
Low percentages of circulating CD8+/CD45RA+ human T lymphocytes expressing β7 integrin correlate with the occurrence of intestinal acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation / Avanzini, M. A.; Maccario, R.; Locatelli, F.; Giebel, S.; Santos, C. D.; Bernardo, M. E.; Pagliara, D.; Montagna, D.; Longo, S.; Amendola, G.; Marconi, M.. - In: EXPERIMENTAL HEMATOLOGY. - ISSN 0301-472X. - 34:10(2006), pp. 1429-1434. [10.1016/j.exphem.2006.06.006]
Low percentages of circulating CD8+/CD45RA+ human T lymphocytes expressing β7 integrin correlate with the occurrence of intestinal acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation
Locatelli F.;
2006
Abstract
Objective: Effector phase of acute graft-versus-host disease (a-GVHD) is mainly mediated by donor-derived, anti-host cytotoxic T cells. T-cell homing into gut-associated lymphoid tissues is ascribed to the α4β7 integrin. We reasoned that development of intestinal a-GVHD might be triggered by recruitment in the intestinal mucosa of circulating, alloreactive, α4β7+ donor T cells. Therefore, we evaluated the correlation existing between circulating β7+ T-lymphocyte subsets early after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and occurrence of a-GVHD. Patients and Methods: Surface expression of β7 integrin on T cells was evaluated by means of direct immunofluorescence, in three-color analysis. Sixty-five patients given allo-HSCT were evaluated: 13 of them experienced intestinal a-GVHD, 14 developed a-GVHD without intestinal involvement, and 38 did not develop a-GVHD. Patients were studied early after initial signs of hematologic reconstitution and before occurrence of a-GVHD. Results: We found a significantly higher absolute number of CD8+ and a significantly lower percentage of CD8+CD45RA+β7+ T cells in patients with intestinal a-GVHD than in patients with a-GVHD without intestinal involvement (p = 0.003 and p = 0.003, respectively) or not experiencing a-GVHD (p = 0.02 and p = 0.002, respectively). In particular, we found that intestinal a-GVHD occurred in over 70% of patients showing an absolute number of CD8+ T cells ≥ 60 × 106/L and a percentage of circulating CD8+CD45RA+β7+ T cells < 35%. Conclusion: Measuring the absolute number of CD8+ T cells and percentage of CD8+CD45RA+β7+ T cells at time of hematologic reconstitution may help identify patients at risk of developing intestinal a-GVHD who could benefit from strategies aimed at hampering alloreactive T-cell homing to intestinal mucosa. © 2006 International Society for Experimental Hematology.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
