The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.

Microgravity-induced cell-to-cell junctional contacts are counteracted by antioxidant compounds in TCAM-2 seminoma cells / Catizone, A.; Morabito, C.; Cammarota, M.; Schiraldi, C.; Scheri, K. C.; Ferranti, F.; Mariggio, M. A.; Ricci, G.. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 10:22(2020), pp. 1-16. [10.3390/app10228289]

Microgravity-induced cell-to-cell junctional contacts are counteracted by antioxidant compounds in TCAM-2 seminoma cells

Catizone A.;
2020

Abstract

The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules.
2020
antioxidant compounds; cell adhesion; microgravity; mitohormesis; TCam-2 seminoma cells
01 Pubblicazione su rivista::01a Articolo in rivista
Microgravity-induced cell-to-cell junctional contacts are counteracted by antioxidant compounds in TCAM-2 seminoma cells / Catizone, A.; Morabito, C.; Cammarota, M.; Schiraldi, C.; Scheri, K. C.; Ferranti, F.; Mariggio, M. A.; Ricci, G.. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 10:22(2020), pp. 1-16. [10.3390/app10228289]
File allegati a questo prodotto
File Dimensione Formato  
Catizone_Microgravity-induced_2020.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 3.42 MB
Formato Adobe PDF
3.42 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1475609
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 3
social impact