The aggregation properties of semaglutide, a lipidated peptide drug agonist of the Glucagon-like peptide 1 receptor recently approved for the treatment of type 2 diabetes, have been investigated by spectroscopic techniques (UV-Vis absorption, steady-state and time-resolved fluorescence, and electronic circular dichroism) and molecular dynamics simulations. We show that in the micromolar concentration region, in aqueous solution, semaglutide is present as monomeric and dimeric species, with a characteristic monomer-to-dimer transition occurring at around 20 μM. The lipid chain stabilizes a globular morphology of the monomer and dimer species, giving rise to a locally well-defined polar outer surface where the lipid and peptide portions are packed to each other. At very long times, these peptide clusters nucleate the growth of larger aggregates characterized by blue luminescence and a β-sheet arrangement of the peptide chains. The understanding of the oligomerization and aggregation potential of peptide candidates is key for the development of long acting and stable drugs.

A spectroscopic and molecular dynamics study on the aggregation process of a long-acting lipidated therapeutic peptide: the case of semaglutide / Venanzi, M.; Savioli, M.; Cimino, R.; Gatto, E.; Palleschi, A.; Ripani, G.; Cicero, D.; Placidi, E.; Orvieto, F.; Bianchi, E.. - In: SOFT MATTER. - ISSN 1744-6848. - 16:44(2020), pp. 10122-10131. [10.1039/d0sm01011a]

A spectroscopic and molecular dynamics study on the aggregation process of a long-acting lipidated therapeutic peptide: the case of semaglutide

Placidi E.;
2020

Abstract

The aggregation properties of semaglutide, a lipidated peptide drug agonist of the Glucagon-like peptide 1 receptor recently approved for the treatment of type 2 diabetes, have been investigated by spectroscopic techniques (UV-Vis absorption, steady-state and time-resolved fluorescence, and electronic circular dichroism) and molecular dynamics simulations. We show that in the micromolar concentration region, in aqueous solution, semaglutide is present as monomeric and dimeric species, with a characteristic monomer-to-dimer transition occurring at around 20 μM. The lipid chain stabilizes a globular morphology of the monomer and dimer species, giving rise to a locally well-defined polar outer surface where the lipid and peptide portions are packed to each other. At very long times, these peptide clusters nucleate the growth of larger aggregates characterized by blue luminescence and a β-sheet arrangement of the peptide chains. The understanding of the oligomerization and aggregation potential of peptide candidates is key for the development of long acting and stable drugs.
2020
Peptides; aggregation processes; type 2 diabetes
01 Pubblicazione su rivista::01a Articolo in rivista
A spectroscopic and molecular dynamics study on the aggregation process of a long-acting lipidated therapeutic peptide: the case of semaglutide / Venanzi, M.; Savioli, M.; Cimino, R.; Gatto, E.; Palleschi, A.; Ripani, G.; Cicero, D.; Placidi, E.; Orvieto, F.; Bianchi, E.. - In: SOFT MATTER. - ISSN 1744-6848. - 16:44(2020), pp. 10122-10131. [10.1039/d0sm01011a]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1463127
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