Agrobacterium rhizogenes (wild-type, strain 1855), when applied to the basal part of microcuttings of walnut (J. regia L.), produced numerous adventitious roots in vitro: 58.6% of rooting was induced in microcuttings in hormone free medium and 62.9% and abundant callus formation in the presence of IBA. A, rhizogenes did not induce rooting when IAA was present in the rooting medium. No explants were induced to root by the treatments with IBA, IAA or in hormone free medium without the presence of A. rhizogenes. Root vascular elements were connected to the microcutting vascular system in the treatment with A. rhizogenes in HFM and were successfully transferred to soil: However, microcuttings treated with A. rhizogenes + IBA showed vascular connections not correctly formed. Southern blots performed using the fragment containing rol genes, as probe, coming from pBIN19::Eco15, provided molecular evidence of the transgenic nature of the roots induced by A. rhizogenes.
Root induction by Agrobacterium rhizogenes in walnut / Emilia, Caboni; Paola, Lauri; Mariagrazia, Tonelli; Falasca, Giuseppina; Carmine, Damiano. - In: PLANT SCIENCE. - ISSN 0168-9452. - STAMPA. - 118:2(1996), pp. 203-208. [10.1016/0168-9452(96)04449-4]
Root induction by Agrobacterium rhizogenes in walnut
FALASCA, Giuseppina;
1996
Abstract
Agrobacterium rhizogenes (wild-type, strain 1855), when applied to the basal part of microcuttings of walnut (J. regia L.), produced numerous adventitious roots in vitro: 58.6% of rooting was induced in microcuttings in hormone free medium and 62.9% and abundant callus formation in the presence of IBA. A, rhizogenes did not induce rooting when IAA was present in the rooting medium. No explants were induced to root by the treatments with IBA, IAA or in hormone free medium without the presence of A. rhizogenes. Root vascular elements were connected to the microcutting vascular system in the treatment with A. rhizogenes in HFM and were successfully transferred to soil: However, microcuttings treated with A. rhizogenes + IBA showed vascular connections not correctly formed. Southern blots performed using the fragment containing rol genes, as probe, coming from pBIN19::Eco15, provided molecular evidence of the transgenic nature of the roots induced by A. rhizogenes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.