Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140(trk-A) and p75(NGFR) molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD ''virgin'' B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific lgG in mice immunized with tetanus toroid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.

Nerve growth factor is an autocrine survival factor for memory B lymphocytes / M., Torcia; L., Bracci Laudiero; M., Lucibello; Nencioni, Lucia; D., Labardi; A., Rubartelli; F., Cozzolino; L., Aloe; E., Garaci. - In: CELL. - ISSN 0092-8674. - STAMPA. - 85:3(1996), pp. 345-356. [10.1016/s0092-8674(00)81113-7]

Nerve growth factor is an autocrine survival factor for memory B lymphocytes

NENCIONI, Lucia;
1996

Abstract

Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140(trk-A) and p75(NGFR) molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD ''virgin'' B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific lgG in mice immunized with tetanus toroid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.
1996
01 Pubblicazione su rivista::01a Articolo in rivista
Nerve growth factor is an autocrine survival factor for memory B lymphocytes / M., Torcia; L., Bracci Laudiero; M., Lucibello; Nencioni, Lucia; D., Labardi; A., Rubartelli; F., Cozzolino; L., Aloe; E., Garaci. - In: CELL. - ISSN 0092-8674. - STAMPA. - 85:3(1996), pp. 345-356. [10.1016/s0092-8674(00)81113-7]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/145579
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