Macrophage colony-stimulating factor (M-CSF) is the main growth factor for mononuclear phagocytes. Responsiveness to growth factors is reduced in the course of functional activation of macrophages, We studied the interference of the macrophage activator interleukin 2 (IL-2) with the response to M-CSF, in macrophages of the M-CSF-dependent murine line BAC-1.2F5, Long-term effects of IL-2 on cell growth were determined, showing that IL-2 reduces the M-CSF-dependent proliferation of macrophages. Short-term effects of IL-2 on the expression of the receptor for M-CSF (M-CSF.R) were characterized in more detail. IL-2 rapidly down-modulated M-CSF.R in a dose-dependent fashion, and interferon-gamma and lipopolysaccharides synergized with IL-2 in this modulation. The IL-2-induced down-modulation of M-CSF.R was shown to require the activity of protein-kinase-C and phospholipase-C. The data are consistent with the hypothesis that the down-modulation of M-CSF.R is a general property of macrophage activators. (C) 1996 Academic Press Limited
Interleukin 2 down-modulates the macrophage colony-stimulating factor receptor in murine macrophages / Persio Dello, Sbarba; Nencioni, Lucia; Danilo, Labardi; Elisabetta, Rovida; Barbara, Caciagli; M. G., Cipolleschi. - In: CYTOKINE. - ISSN 1043-4666. - STAMPA. - 8:6(1996), pp. 488-494. [10.1006/cyto.1996.0066]
Interleukin 2 down-modulates the macrophage colony-stimulating factor receptor in murine macrophages
NENCIONI, Lucia;
1996
Abstract
Macrophage colony-stimulating factor (M-CSF) is the main growth factor for mononuclear phagocytes. Responsiveness to growth factors is reduced in the course of functional activation of macrophages, We studied the interference of the macrophage activator interleukin 2 (IL-2) with the response to M-CSF, in macrophages of the M-CSF-dependent murine line BAC-1.2F5, Long-term effects of IL-2 on cell growth were determined, showing that IL-2 reduces the M-CSF-dependent proliferation of macrophages. Short-term effects of IL-2 on the expression of the receptor for M-CSF (M-CSF.R) were characterized in more detail. IL-2 rapidly down-modulated M-CSF.R in a dose-dependent fashion, and interferon-gamma and lipopolysaccharides synergized with IL-2 in this modulation. The IL-2-induced down-modulation of M-CSF.R was shown to require the activity of protein-kinase-C and phospholipase-C. The data are consistent with the hypothesis that the down-modulation of M-CSF.R is a general property of macrophage activators. (C) 1996 Academic Press LimitedI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
