This study investigates, for the first time, dual C-Cl isotope fractionation during anaerobic biodegradation of 1,2-dichloroethane (1,2-DCA) via dihaloelimination by Dehalococcoides and Dehalogenimonas-containing enrichment cultures. Isotopic fractionation of 1,2-DCA (εbulkC and εbulkCl) for Dehalococcoides (-33.0 ± 0.4‰ and -5.1 ± 0.1‰) and Dehalogenimonas-containing microcosms (-23 ± 2‰ and -12.0 ± 0.8‰) resulted in distinctly different dual element C-Cl isotope correlations (∇ = Δδ13C/Δδ37Cl ≈ εbulkC/εbulkCl), 6.8 ± 0.2 and 1.89 ± 0.02, respectively. Determined isotope effects and detected products suggest that the difference on the obtained ∇ values for biodihaloelimination could be associated with a different mode of concerted bond cleavage rather than two different reaction pathways (i.e., stepwise vs concerted). ∇ values of 1,2-DCA were, for the first time, determined in two field sites under reducing conditions (2.1 ± 0.1 and 2.2 ± 2.9). They were similar to the one obtained for the Dehalogenimonas-containing microcosms (1.89 ± 0.02) and very different from those reported for aerobic degradation pathways in a previous laboratory study (7.6 ± 0.1 and 0.78 ± 0.03). Thus, this study illustrates the potential of a dual isotope analysis to differentiate between aerobic and anaerobic biodegradation pathways of 1,2-DCA in the field and suggests that this approach might also be used to characterize dihaloelimination of 1,2-DCA by different bacteria, which needs to be confirmed in future studies.

Distinct dual C-Cl Isotope Ffactionation patterns during anaerobic biodegradation of 1,2-Dichloroethane. Potential to characterize microbial degradation in the field / Palau, J.; Yu, R.; Hatijah Mortan, S.; Shouakar-Stash, O.; Rosell, M.; Freedman, D. L.; Sbarbati, C.; Fiorenza, S.; Aravena, R.; Marco-Urrea, E.; Elsner, M.; Soler, A.; Hunkeler, D.. - In: ENVIRONMENTAL SCIENCE & TECHNOLOGY. - ISSN 0013-936X. - 51:5(2017), pp. 2685-2694. [10.1021/acs.est.6b04998]

Distinct dual C-Cl Isotope Ffactionation patterns during anaerobic biodegradation of 1,2-Dichloroethane. Potential to characterize microbial degradation in the field

Sbarbati C.;Aravena R.;
2017

Abstract

This study investigates, for the first time, dual C-Cl isotope fractionation during anaerobic biodegradation of 1,2-dichloroethane (1,2-DCA) via dihaloelimination by Dehalococcoides and Dehalogenimonas-containing enrichment cultures. Isotopic fractionation of 1,2-DCA (εbulkC and εbulkCl) for Dehalococcoides (-33.0 ± 0.4‰ and -5.1 ± 0.1‰) and Dehalogenimonas-containing microcosms (-23 ± 2‰ and -12.0 ± 0.8‰) resulted in distinctly different dual element C-Cl isotope correlations (∇ = Δδ13C/Δδ37Cl ≈ εbulkC/εbulkCl), 6.8 ± 0.2 and 1.89 ± 0.02, respectively. Determined isotope effects and detected products suggest that the difference on the obtained ∇ values for biodihaloelimination could be associated with a different mode of concerted bond cleavage rather than two different reaction pathways (i.e., stepwise vs concerted). ∇ values of 1,2-DCA were, for the first time, determined in two field sites under reducing conditions (2.1 ± 0.1 and 2.2 ± 2.9). They were similar to the one obtained for the Dehalogenimonas-containing microcosms (1.89 ± 0.02) and very different from those reported for aerobic degradation pathways in a previous laboratory study (7.6 ± 0.1 and 0.78 ± 0.03). Thus, this study illustrates the potential of a dual isotope analysis to differentiate between aerobic and anaerobic biodegradation pathways of 1,2-DCA in the field and suggests that this approach might also be used to characterize dihaloelimination of 1,2-DCA by different bacteria, which needs to be confirmed in future studies.
2017
1,2 DCA; isotope fractionation; Dehalococcoides
01 Pubblicazione su rivista::01a Articolo in rivista
Distinct dual C-Cl Isotope Ffactionation patterns during anaerobic biodegradation of 1,2-Dichloroethane. Potential to characterize microbial degradation in the field / Palau, J.; Yu, R.; Hatijah Mortan, S.; Shouakar-Stash, O.; Rosell, M.; Freedman, D. L.; Sbarbati, C.; Fiorenza, S.; Aravena, R.; Marco-Urrea, E.; Elsner, M.; Soler, A.; Hunkeler, D.. - In: ENVIRONMENTAL SCIENCE & TECHNOLOGY. - ISSN 0013-936X. - 51:5(2017), pp. 2685-2694. [10.1021/acs.est.6b04998]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1453065
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