The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and maintenance of chromosomal silencing. Here, we introduce a new version of our algorithm catRAPID to investigate Xist associations with a number of proteins involved in epigenetic regulation, nuclear scaffolding, transcription and splicing processes. Our method correctly identifies binding regions and affinities of protein interactions, providing a powerful theoretical framework for the study of X-chromosome inactivation and other events mediated by ribonucleoprotein associations. © 2012 The Author(s). Published by Oxford University Press.
X-inactivation: quantitative predictions of protein interactions in the Xist network / Agostini, F.; Cirillo, D.; Bolognesi, B.; Tartaglia, G. G.. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - 41:1(2013), p. e31. [10.1093/nar/gks968]
X-inactivation: quantitative predictions of protein interactions in the Xist network
Tartaglia G. G.
2013
Abstract
The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and maintenance of chromosomal silencing. Here, we introduce a new version of our algorithm catRAPID to investigate Xist associations with a number of proteins involved in epigenetic regulation, nuclear scaffolding, transcription and splicing processes. Our method correctly identifies binding regions and affinities of protein interactions, providing a powerful theoretical framework for the study of X-chromosome inactivation and other events mediated by ribonucleoprotein associations. © 2012 The Author(s). Published by Oxford University Press.File | Dimensione | Formato | |
---|---|---|---|
Agostini_X-inactivation_2013.pdf
accesso aperto
Tipologia:
Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza:
Creative commons
Dimensione
4.4 MB
Formato
Adobe PDF
|
4.4 MB | Adobe PDF |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.