The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and maintenance of chromosomal silencing. Here, we introduce a new version of our algorithm catRAPID to investigate Xist associations with a number of proteins involved in epigenetic regulation, nuclear scaffolding, transcription and splicing processes. Our method correctly identifies binding regions and affinities of protein interactions, providing a powerful theoretical framework for the study of X-chromosome inactivation and other events mediated by ribonucleoprotein associations. © 2012 The Author(s). Published by Oxford University Press.

X-inactivation: quantitative predictions of protein interactions in the Xist network / Agostini, F.; Cirillo, D.; Bolognesi, B.; Tartaglia, G. G.. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - 41:1(2013), p. e31. [10.1093/nar/gks968]

X-inactivation: quantitative predictions of protein interactions in the Xist network

Tartaglia G. G.
2013

Abstract

The transcriptional silencing of one of the female X-chromosomes is a finely regulated process that requires accumulation in cis of the long non-coding RNA X-inactive-specific transcript (Xist) followed by a series of epigenetic modifications. Little is known about the molecular machinery regulating initiation and maintenance of chromosomal silencing. Here, we introduce a new version of our algorithm catRAPID to investigate Xist associations with a number of proteins involved in epigenetic regulation, nuclear scaffolding, transcription and splicing processes. Our method correctly identifies binding regions and affinities of protein interactions, providing a powerful theoretical framework for the study of X-chromosome inactivation and other events mediated by ribonucleoprotein associations. © 2012 The Author(s). Published by Oxford University Press.
2013
Animals; bindings; enhancer of Zeste homolog 2 protein; female; heterogeneous-nuclear ribonucleoprotein U; matrix attachment region binding proteins; mice; nuclear proteins; polycomb repressive complex 2; RNA, long noncoding; RNA-binding proteins; repetitive sequences, nucleic acid; serine-arginine splicing factors; YY1 transcription factor; algorithms; X chromosome inactivation
01 Pubblicazione su rivista::01a Articolo in rivista
X-inactivation: quantitative predictions of protein interactions in the Xist network / Agostini, F.; Cirillo, D.; Bolognesi, B.; Tartaglia, G. G.. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - 41:1(2013), p. e31. [10.1093/nar/gks968]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1451739
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