Recent studies describing the detection of SARS-CoV-2 RNA in pools of 5 to 32 samples reported false negative rates up to 10% for large groups, suggesting that smaller sample pools are a good compromise to increase sample processing capacity while maintaining test reliability. Since 5-sample pools were shown to efficiently detect SARS-CoV-2 RNA in RT-PCR assays, we chose to test and validate this approach using a highthroughput RNA extraction and amplification platform.

Validation of a small-size pooling approach targeting hospital surveillance of SARS-CoV-2 infection / Petrucca, Andrea; Borro, Marina; Lionetto, Luana; Gentile, Giovanna; Alari, Antonella; Simmaco, Maurizio; Santino, Iolanda. - In: INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY. - ISSN 0899-823X. - (2020), pp. 1-2. [10.1017/ice.2020.380]

Validation of a small-size pooling approach targeting hospital surveillance of SARS-CoV-2 infection

Marina Borro;Giovanna Gentile;Maurizio Simmaco;Iolanda Santino
2020

Abstract

Recent studies describing the detection of SARS-CoV-2 RNA in pools of 5 to 32 samples reported false negative rates up to 10% for large groups, suggesting that smaller sample pools are a good compromise to increase sample processing capacity while maintaining test reliability. Since 5-sample pools were shown to efficiently detect SARS-CoV-2 RNA in RT-PCR assays, we chose to test and validate this approach using a highthroughput RNA extraction and amplification platform.
2020
SARS-COV-2; pooling; surveillance
01 Pubblicazione su rivista::01f Lettera, Nota
Validation of a small-size pooling approach targeting hospital surveillance of SARS-CoV-2 infection / Petrucca, Andrea; Borro, Marina; Lionetto, Luana; Gentile, Giovanna; Alari, Antonella; Simmaco, Maurizio; Santino, Iolanda. - In: INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY. - ISSN 0899-823X. - (2020), pp. 1-2. [10.1017/ice.2020.380]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1450926
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