Resistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum beta-lactamases (ESBLs). Clear identification of risk factors for ESBLs-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the antibacterial susceptibility and ESBLs pattern of diarrhogenic Escherichia coli (E. coli) strains isolated from adult patients. In the present study, diarrheogenic E. coli strains were isolated from 54 patients from the University of Medical Sciences hospitals in Shiraz. Antimicrobial susceptibility testing was done by disk diffusion method by CLSI criteria. The presence of bla(TEM), bla(SHV) and bla(CTX-M) genes was investigated by PCR using designated primers. The prevalence of ESBLs-producer E. coli strains was 12.96%. Antimicrobial resistance testing showed a high resistance to cefexime, trimethoprim-sulfamethoxazole, ampicillin and penicillin. Overall, beta-lactamase genes were identified in 52 (96.30%) isolates which were identified as 45 (83.33%) bla(TEM), 17 (31.48%) bla(SHV) and 11 (20.37%) bla(CTX-M). ESBLs-producer E. coli is very prevalent in diarrheogenic strains isolated from adult patients. Also, this study clearly showed that the blaT(EM) gene for ESBLs-producer E. coli was widespread in Iran.
Molecular epidemiology of ESBLs genes and multi-drug resistance in diarrheagenic Escherichia Coli strains isolated from adults in Iran / Sadegh, Ghorbani-Dalini; Mohammad, Kargar; Abbas, Doosti; Pejman, Abbasi; Sarsharjeryandeh, Meysam. - In: IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH. - ISSN 1735-0328. - 14:4(2015), pp. 1257-1262.
Molecular epidemiology of ESBLs genes and multi-drug resistance in diarrheagenic Escherichia Coli strains isolated from adults in Iran
Meysam Sarshar
Ultimo
2015
Abstract
Resistance to oxyimino cephalosporins antibiotics in Enterobacteriaceae is primarily done by the extended spectrum beta-lactamases (ESBLs). Clear identification of risk factors for ESBLs-producing infections is necessary. Therefore, efficient strategies can be developed to decrease outbreak of these infections. The aim of this study was to determine the antibacterial susceptibility and ESBLs pattern of diarrhogenic Escherichia coli (E. coli) strains isolated from adult patients. In the present study, diarrheogenic E. coli strains were isolated from 54 patients from the University of Medical Sciences hospitals in Shiraz. Antimicrobial susceptibility testing was done by disk diffusion method by CLSI criteria. The presence of bla(TEM), bla(SHV) and bla(CTX-M) genes was investigated by PCR using designated primers. The prevalence of ESBLs-producer E. coli strains was 12.96%. Antimicrobial resistance testing showed a high resistance to cefexime, trimethoprim-sulfamethoxazole, ampicillin and penicillin. Overall, beta-lactamase genes were identified in 52 (96.30%) isolates which were identified as 45 (83.33%) bla(TEM), 17 (31.48%) bla(SHV) and 11 (20.37%) bla(CTX-M). ESBLs-producer E. coli is very prevalent in diarrheogenic strains isolated from adult patients. Also, this study clearly showed that the blaT(EM) gene for ESBLs-producer E. coli was widespread in Iran.File | Dimensione | Formato | |
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