Objectives: Basic Fibroblastic Growth Factor (bFGF) is a powerful mitogen for smooth muscle cells and has been implicated in the genesis of Myointimal hyperplasia. The aim of this study was to determine the release of bFGF by veins in different haemodynamic conditions. Design and setting: Laboratory animal study. Materials: In 39 Lewis rats, a 1 cm long segment of inferior vena cava was inserted at the level of the abdominal aorta. The segments of inferior vena cava were obtained from syngenic Lewis rats. Arterial Vein Grafts (AVG) were harvested after 4 weeks (AVG4) and 12 weeks (AVG 12). In 16 animals the arterial vein grafts were explanted 4 weeks after the initial operation and reimplanted (Reimplanted Vein Grafts: RVG) in syngenic Lewis rats as venous-venous bypass grafts at the level of the left iliac vein and harvested after 2 weeks (RVG 2) and 8 weeks (AVG 8). Outcome measures: The tissue was studied in organ culture in a serum-free system for (1) release of bFGF (immunoassay) and (2) mitogenic activity of the conditioned media. Scanning electron and light microscopy studies were also performed. Results: bFGF release by veins increased significantly (p < 0.01) when veins were inserted in the arterial circulation, and decreased significantly (p < 0.01) when grafts where reimplanted in the venous system. bFGF release (ng/cm2):. © 1995 W. B. Saunders Company Ltd. All rights reserved.

bFGF release is dependent on flow conditions in experimental vein grafts / Lepidi, S.; Sterpetti, A. V.; Cucina, A.; Di Carlo, A.; Patrizi, A. L.; Palumbo, R.; Bernucci, P.; Santoro-D'Angelo, L.; Cavallaro, A.. - In: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY. - ISSN 1078-5884. - 10:4(1995), pp. 450-458. [10.1016/S1078-5884(05)80168-6]

bFGF release is dependent on flow conditions in experimental vein grafts

Sterpetti A. V.
Conceptualization
;
Cucina A.;Santoro-D'Angelo L.;Cavallaro A.
1995

Abstract

Objectives: Basic Fibroblastic Growth Factor (bFGF) is a powerful mitogen for smooth muscle cells and has been implicated in the genesis of Myointimal hyperplasia. The aim of this study was to determine the release of bFGF by veins in different haemodynamic conditions. Design and setting: Laboratory animal study. Materials: In 39 Lewis rats, a 1 cm long segment of inferior vena cava was inserted at the level of the abdominal aorta. The segments of inferior vena cava were obtained from syngenic Lewis rats. Arterial Vein Grafts (AVG) were harvested after 4 weeks (AVG4) and 12 weeks (AVG 12). In 16 animals the arterial vein grafts were explanted 4 weeks after the initial operation and reimplanted (Reimplanted Vein Grafts: RVG) in syngenic Lewis rats as venous-venous bypass grafts at the level of the left iliac vein and harvested after 2 weeks (RVG 2) and 8 weeks (AVG 8). Outcome measures: The tissue was studied in organ culture in a serum-free system for (1) release of bFGF (immunoassay) and (2) mitogenic activity of the conditioned media. Scanning electron and light microscopy studies were also performed. Results: bFGF release by veins increased significantly (p < 0.01) when veins were inserted in the arterial circulation, and decreased significantly (p < 0.01) when grafts where reimplanted in the venous system. bFGF release (ng/cm2):. © 1995 W. B. Saunders Company Ltd. All rights reserved.
1995
ARTERIAL VEIN GRAFT; SHEAR STRESS; FIBROBLASTIC GROWTH FACTOR; MYOINTIMAL HYPERPLASIA; Animals; Aorta, Abdominal; Culture Media, Conditioned; Culture Media, Serum-Free; Enzyme-Linked Immunosorbent Assay; Fibroblast Growth Factor 2; Hyperplasia; Iliac Vein; Male; Microscopy, Electron, Scanning; Mitogens; Muscle, Smooth, Vascular; Organ Culture Techniques; Rats; Rats, Inbred Lew; Replantation; Time Factors; Tunica Intima; Vena Cava, Inferior
01 Pubblicazione su rivista::01a Articolo in rivista
bFGF release is dependent on flow conditions in experimental vein grafts / Lepidi, S.; Sterpetti, A. V.; Cucina, A.; Di Carlo, A.; Patrizi, A. L.; Palumbo, R.; Bernucci, P.; Santoro-D'Angelo, L.; Cavallaro, A.. - In: EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY. - ISSN 1078-5884. - 10:4(1995), pp. 450-458. [10.1016/S1078-5884(05)80168-6]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1442025
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