Treatment of cells with interferons (IFNs) induces resistance to virus infection. The 2'-5'oligo A (2-5A) synthetase/RNase L is one of the pathways leading to translation inhibition induced by IFN treatment. A murine cDNA encoding the 43-kDa 2-5A synthetase was cloned and sequenced. NIH-3T3 cell clones transfected with this cDNA expressed the enzymatic activity to various extents and exhibited resistance to encephalomyocarditis virus (EMCV) but not to vesicular stomatitis virus replication. The specific resistance to EMCV can be attributed to 2-5A synthetase.
A full length murine 2 5A synthetase cDNA transfected in NIH 3T3 cells impairs EMCV but not VSV replication / Coccia, E. M.; Romeo, Giovanna; Nissim, A.; Marziali, G.; Albertini, R.; Affabris, E.; Battistini, A.; Fiorucci, G.; Orsatti, R.; Rossi, G. B.; AND CHEBATH, J.. - In: VIROLOGY. - ISSN 0042-6822. - STAMPA. - 179:(1990), pp. 228-233. [10.1016/0042-6822(90)90292-Y]
A full length murine 2 5A synthetase cDNA transfected in NIH 3T3 cells impairs EMCV but not VSV replication.
ROMEO, Giovanna;
1990
Abstract
Treatment of cells with interferons (IFNs) induces resistance to virus infection. The 2'-5'oligo A (2-5A) synthetase/RNase L is one of the pathways leading to translation inhibition induced by IFN treatment. A murine cDNA encoding the 43-kDa 2-5A synthetase was cloned and sequenced. NIH-3T3 cell clones transfected with this cDNA expressed the enzymatic activity to various extents and exhibited resistance to encephalomyocarditis virus (EMCV) but not to vesicular stomatitis virus replication. The specific resistance to EMCV can be attributed to 2-5A synthetase.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
