The detection of atypical Kinetoplastida in vertebrate hosts and vectors might suggest unexpected host‐parasite contacts. Aside to major vectors of Leishmania (Leishmania) infantum in Italy (e.g. Phlebotomus perniciosus and Phlebotomus perfiliewi), the sand fly fauna also includes Sergentomyia minuta, herpetophilic and proven vector of Leishmania (Sauroleishmania) tarentolae, in which records of blood meal on mammals and detection of L. infantum DNA are increasing. This study was conducted in Central Italy aiming to molecularly detect potential atypical Leishmania host‐vector contacts. Detection of Leishmania spp. DNA was performed by polymerase chain reaction (SSU rRNA, ITS1 targets) on field‐collected sand fly females (N = 344), blood samples from humans (N = 185) and dogs (N = 125). Blood meal identification was also performed on engorged sand flies. Leishmania spp. DNA was found in 13.1% sand flies, 3.7% humans and 14.4% dogs. Sequence analysis identified L. infantum in S. minuta (4.4%), P. perniciosus (9.1%), humans (2.2%) and dogs (14.4%). Leishmania tarentolae was detected in S. minuta (12.6%), P. perfiliewi (6.6%) and human (1.6%) samples. Of 28 S. minuta examined for blood meal, 3.6 and 21.4% scored positive for human and lizard DNA, respectively. These results indicate the importance of one‐health approach to explore new potential routes of transmission of leishmaniasis involving S. minuta.

Molecular detection of Leishmania (Sauroleishmania) tarentolae in human blood and Leishmania (Leishmania) infantum in Sergentomyia minuta. Unexpected host-parasite contacts / Pombi, M.; Giacomi, A.; Barlozzari, G.; Mendoza-Roldan, J.; Macrì, G.; Otranto, D.; Gabrielli, S.. - In: MEDICAL AND VETERINARY ENTOMOLOGY. - ISSN 0269-283X. - (2020), pp. 1-6. [10.1111/mve.12464]

Molecular detection of Leishmania (Sauroleishmania) tarentolae in human blood and Leishmania (Leishmania) infantum in Sergentomyia minuta. Unexpected host-parasite contacts

Pombi, M.
Primo
;
Giacomi, A.
Secondo
;
Gabrielli, S.
Ultimo
2020

Abstract

The detection of atypical Kinetoplastida in vertebrate hosts and vectors might suggest unexpected host‐parasite contacts. Aside to major vectors of Leishmania (Leishmania) infantum in Italy (e.g. Phlebotomus perniciosus and Phlebotomus perfiliewi), the sand fly fauna also includes Sergentomyia minuta, herpetophilic and proven vector of Leishmania (Sauroleishmania) tarentolae, in which records of blood meal on mammals and detection of L. infantum DNA are increasing. This study was conducted in Central Italy aiming to molecularly detect potential atypical Leishmania host‐vector contacts. Detection of Leishmania spp. DNA was performed by polymerase chain reaction (SSU rRNA, ITS1 targets) on field‐collected sand fly females (N = 344), blood samples from humans (N = 185) and dogs (N = 125). Blood meal identification was also performed on engorged sand flies. Leishmania spp. DNA was found in 13.1% sand flies, 3.7% humans and 14.4% dogs. Sequence analysis identified L. infantum in S. minuta (4.4%), P. perniciosus (9.1%), humans (2.2%) and dogs (14.4%). Leishmania tarentolae was detected in S. minuta (12.6%), P. perfiliewi (6.6%) and human (1.6%) samples. Of 28 S. minuta examined for blood meal, 3.6 and 21.4% scored positive for human and lizard DNA, respectively. These results indicate the importance of one‐health approach to explore new potential routes of transmission of leishmaniasis involving S. minuta.
2020
kinetoplastida; trypanosomatidae; italy; lazio region; leishmaniasis; lizard; molecular detection; one health; zoonosis
01 Pubblicazione su rivista::01a Articolo in rivista
Molecular detection of Leishmania (Sauroleishmania) tarentolae in human blood and Leishmania (Leishmania) infantum in Sergentomyia minuta. Unexpected host-parasite contacts / Pombi, M.; Giacomi, A.; Barlozzari, G.; Mendoza-Roldan, J.; Macrì, G.; Otranto, D.; Gabrielli, S.. - In: MEDICAL AND VETERINARY ENTOMOLOGY. - ISSN 0269-283X. - (2020), pp. 1-6. [10.1111/mve.12464]
File allegati a questo prodotto
File Dimensione Formato  
Pombi_Molecular_2020.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 676.21 kB
Formato Adobe PDF
676.21 kB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1435650
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 32
  • ???jsp.display-item.citation.isi??? 26
social impact