The presence of interferon (IFN) receptors on mouse Friend leukemia cells (FLC) has been investigated in binding experiments with highly purified 125I-labeled mouse alpha/beta IFN. Both IFN-resistant clones and wild-type IFN-sensitive FLC showed a specific saturable binding site for mouse IFN with a similar affinity constant. In contrast to IFN-sensitive FLC, IFN-resistant FLC variants were not inducible by IFN for double-stranded RNA-dependent 2-5A synthetase activity.
2-5A synthetase activity does not increase in interferon resistent Friend leukemia cell variants treated with alpha/beta interferon despite the presence of high affinity interferon receptor sites / Affabris, E.; Romeo, Giovanna; Belardelli, F.; Jemma, C.; Mechti, N.; Gresser, I.; Rossi, G. B.. - In: VIROLOGY. - ISSN 0042-6822. - STAMPA. - 125::(1983), pp. 508-512.
2-5A synthetase activity does not increase in interferon resistent Friend leukemia cell variants treated with alpha/beta interferon despite the presence of high affinity interferon receptor sites.
ROMEO, Giovanna;
1983
Abstract
The presence of interferon (IFN) receptors on mouse Friend leukemia cells (FLC) has been investigated in binding experiments with highly purified 125I-labeled mouse alpha/beta IFN. Both IFN-resistant clones and wild-type IFN-sensitive FLC showed a specific saturable binding site for mouse IFN with a similar affinity constant. In contrast to IFN-sensitive FLC, IFN-resistant FLC variants were not inducible by IFN for double-stranded RNA-dependent 2-5A synthetase activity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
