MicroRNAs (miRs) have emerged as biomarkers of migraine disease in both adults and children. In this study we evaluated the expression of hsa-miR-34a-5p and hsa-miR-375 in serum and saliva of young subjects (age 11 +/- 3.467 years) with migraine without aura (MWA), while some underwent pharmacological treatment, and healthy young subjects were used as controls. miRs were determined using the qRT-PCR method, and gene targets of hsa-miR-34a-5p and hsa-miR-375 linked to pain-migraine were found by in silico analysis. qRT-PCR revealed comparable levels of hsa-miRs in both blood and saliva. Higher expression of hsa-miR-34a-5p and hsa-miR-375 was detected in saliva of untreated MWAs compared to healthy subjects (hsa-miR-34a-5p: p < 0.05; hsa-miR-375 p < 0.01). Furthermore, in MWA treated subjects, a significant decrease of hsa-miR-34a-5p and of hsa-miR-375 was documented in saliva and blood compared to MWA untreated ones. Altogether, these findings suggested thathsa-miR-34a-5p and hsa-miR-375 are expressed equally in blood and saliva and that they could be a useful biomarker of disease and of drug efficacy in patients with MWA.

Hsa-miR-34a-5p and hsa-miR-375 as biomarkers for monitoring the effects of drug treatment for migraine pain in children and adolescents: A Pilot study / Gallelli, L; Cione, E; Peltrone, F; Siviglia, S; Verano, A; Chirchiglia, D; Zampogna, S; Guidetti, V; Sammartino, L; Montana, A; Caroleo, Mc; De Sarro, G; Di Mizio, G. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 8:7(2019), pp. 1-11. [10.3390/jcm8070928]

Hsa-miR-34a-5p and hsa-miR-375 as biomarkers for monitoring the effects of drug treatment for migraine pain in children and adolescents: A Pilot study

Peltrone, F;Guidetti, V;Montana, A;De Sarro, G;Di Mizio, G
2019

Abstract

MicroRNAs (miRs) have emerged as biomarkers of migraine disease in both adults and children. In this study we evaluated the expression of hsa-miR-34a-5p and hsa-miR-375 in serum and saliva of young subjects (age 11 +/- 3.467 years) with migraine without aura (MWA), while some underwent pharmacological treatment, and healthy young subjects were used as controls. miRs were determined using the qRT-PCR method, and gene targets of hsa-miR-34a-5p and hsa-miR-375 linked to pain-migraine were found by in silico analysis. qRT-PCR revealed comparable levels of hsa-miRs in both blood and saliva. Higher expression of hsa-miR-34a-5p and hsa-miR-375 was detected in saliva of untreated MWAs compared to healthy subjects (hsa-miR-34a-5p: p < 0.05; hsa-miR-375 p < 0.01). Furthermore, in MWA treated subjects, a significant decrease of hsa-miR-34a-5p and of hsa-miR-375 was documented in saliva and blood compared to MWA untreated ones. Altogether, these findings suggested thathsa-miR-34a-5p and hsa-miR-375 are expressed equally in blood and saliva and that they could be a useful biomarker of disease and of drug efficacy in patients with MWA.
2019
biomarkers; miRs; saliva; blood; pain-migraine; drug treatment; clinical risk management
01 Pubblicazione su rivista::01a Articolo in rivista
Hsa-miR-34a-5p and hsa-miR-375 as biomarkers for monitoring the effects of drug treatment for migraine pain in children and adolescents: A Pilot study / Gallelli, L; Cione, E; Peltrone, F; Siviglia, S; Verano, A; Chirchiglia, D; Zampogna, S; Guidetti, V; Sammartino, L; Montana, A; Caroleo, Mc; De Sarro, G; Di Mizio, G. - In: JOURNAL OF CLINICAL MEDICINE. - ISSN 2077-0383. - 8:7(2019), pp. 1-11. [10.3390/jcm8070928]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1429248
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