BACKGROUND: Microalgae are a promising resource to produce carotenoids for food/feed applications. However, energy-intensive pre-treatments such as drying and cell destruction can hinder the sustainability of the process. Direct extraction from wet biomass may reduce energy consumption, but the residual water can reduce the effectiveness of the organic solvents used for the extraction. In this work, the extraction of lutein and β-carotene from wet untreated microalgal biomass is investigated. The process includes a sequence of successive extraction stages with methanol. RESULTS: We show that the sequential extraction can effectively overcome the reduced extraction efficiency induced by the residual water in the biomass. The biomass was dehydrated during the first extraction stage, which increased the efficiency starting from the second stage. This allowed an extraction yield of >95% to be attained for both lutein and β-carotene after five extraction stages. A mathematical model was developed by coupling mass balances with a linear equilibrium model, satisfactorily describing the partition of the two carotenoids between the solid and the solvent phase. By fitting the model to the data obtained by the first extraction stage, liquid–solid partition coefficients (Kd) equal to 2.0 × 10−3 and 1.1 × 10−4 were estimated for lutein and β-carotene, respectively. The Kd of both carotenoids increased to ≈10−2 during the six subsequent extraction stages without water. CONCLUSIONS: Direct solvent extraction from wet untreated microalgal biomass is a promising strategy to produce carotenoids. The described model can provide guidelines to design industrial processes.

Sequential extraction of lutein and β‐carotene from wet microalgal biomass / Di Caprio, Fabrizio; Altimari, Pietro; Pagnanelli, Francesca. - In: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. - ISSN 1097-4660. - 95:11(2020), pp. 3024-3033. [10.1002/jctb.6464]

Sequential extraction of lutein and β‐carotene from wet microalgal biomass

Fabrizio Di Caprio
Primo
;
Pietro Altimari
Secondo
;
Francesca Pagnanelli
Ultimo
2020

Abstract

BACKGROUND: Microalgae are a promising resource to produce carotenoids for food/feed applications. However, energy-intensive pre-treatments such as drying and cell destruction can hinder the sustainability of the process. Direct extraction from wet biomass may reduce energy consumption, but the residual water can reduce the effectiveness of the organic solvents used for the extraction. In this work, the extraction of lutein and β-carotene from wet untreated microalgal biomass is investigated. The process includes a sequence of successive extraction stages with methanol. RESULTS: We show that the sequential extraction can effectively overcome the reduced extraction efficiency induced by the residual water in the biomass. The biomass was dehydrated during the first extraction stage, which increased the efficiency starting from the second stage. This allowed an extraction yield of >95% to be attained for both lutein and β-carotene after five extraction stages. A mathematical model was developed by coupling mass balances with a linear equilibrium model, satisfactorily describing the partition of the two carotenoids between the solid and the solvent phase. By fitting the model to the data obtained by the first extraction stage, liquid–solid partition coefficients (Kd) equal to 2.0 × 10−3 and 1.1 × 10−4 were estimated for lutein and β-carotene, respectively. The Kd of both carotenoids increased to ≈10−2 during the six subsequent extraction stages without water. CONCLUSIONS: Direct solvent extraction from wet untreated microalgal biomass is a promising strategy to produce carotenoids. The described model can provide guidelines to design industrial processes.
2020
algae; downstream; extraction; mathematical modelling; solid/liquid separation; industrial microbiology
01 Pubblicazione su rivista::01a Articolo in rivista
Sequential extraction of lutein and β‐carotene from wet microalgal biomass / Di Caprio, Fabrizio; Altimari, Pietro; Pagnanelli, Francesca. - In: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. - ISSN 1097-4660. - 95:11(2020), pp. 3024-3033. [10.1002/jctb.6464]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1428372
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