The increasing interest toward biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. To this regard, recently the use of pulsed electric fields to trigger release across phospholipid membranes of liposomes has been numerically studied, for a deeper understanding of the phenomena at the molecular scale. Aim of this work is to give an experimental validation of the feasibility to control the release from liposome vesicles, using nanosecond pulsed electric fields characterized by a 10 ns duration and intensity in the order of MV/m. The results are supported by multiphysics simulations which consider the coupling of three physics (electromagnetics, thermal and pore kinetics) in order to explain the occurring physical interactions at the microscopic level and provide useful information on the characteristics of the train of pulses needed to obtain quantitative results in terms of liposome electropermeabilization. Finally, a complete characterization of the exposure system is also provided to support the reliability and validity of the study.

Proof-of-concept of electrical activation of liposome nanocarriers: from dry to wet experiments / Caramazza, Laura; Nardoni, Martina; De Angelis, Annalisa; Paolicelli, Patrizia; Liberti, Micaela; Apollonio, Francesca; Petralito, Stefania. - In: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY. - ISSN 2296-4185. - 8:(2020). [10.3389/fbioe.2020.00819]

Proof-of-concept of electrical activation of liposome nanocarriers: from dry to wet experiments

Laura Caramazza;Martina Nardoni;Patrizia Paolicelli;Micaela Liberti;Francesca Apollonio
;
Stefania Petralito
2020

Abstract

The increasing interest toward biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. To this regard, recently the use of pulsed electric fields to trigger release across phospholipid membranes of liposomes has been numerically studied, for a deeper understanding of the phenomena at the molecular scale. Aim of this work is to give an experimental validation of the feasibility to control the release from liposome vesicles, using nanosecond pulsed electric fields characterized by a 10 ns duration and intensity in the order of MV/m. The results are supported by multiphysics simulations which consider the coupling of three physics (electromagnetics, thermal and pore kinetics) in order to explain the occurring physical interactions at the microscopic level and provide useful information on the characteristics of the train of pulses needed to obtain quantitative results in terms of liposome electropermeabilization. Finally, a complete characterization of the exposure system is also provided to support the reliability and validity of the study.
2020
nanosecond pulsed electric fields; liposome vesicles; controlled release; electroporation; electropermeabilization; exposure systems; multiphysics modeling
01 Pubblicazione su rivista::01a Articolo in rivista
Proof-of-concept of electrical activation of liposome nanocarriers: from dry to wet experiments / Caramazza, Laura; Nardoni, Martina; De Angelis, Annalisa; Paolicelli, Patrizia; Liberti, Micaela; Apollonio, Francesca; Petralito, Stefania. - In: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY. - ISSN 2296-4185. - 8:(2020). [10.3389/fbioe.2020.00819]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1422639
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