P5.10 - Nucleolar variant of ErbB3 regulates RNA polymerase I activity Donatella Ponti. Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy The nucleolus is the primary site of the transcription of the ribosomal genes. Rapid growth and division of cells, including tumor cells, is correlated with intensive protein biosynthesis. We have identified a short variant of ErbB3 that localizes in the nucleolus of several cell lines, and, in this compartment it interacts directly with UBF a major transcriptional factor that modulates RNA Pol I activity. Interestingly the presence of this short variant in the nucleolus is inversed correlated with the level of pre-rRNA.The localization of ErbB3 in the cell is under neuregulin control. By performing experiments of immunoprecipitation with anti ErbB3 antibodies, following by immunoblotting we have demonstrated that the interaction between the ErbB3 variant and UBF depends by neuregulin stimulation.ErbB3 receptor represent an emerging cancer target because it plays an important role in drug resistance therapies: interfere with the function of this ErbB3 nucleolar variant could represent an innovative approach to counteract cancer progression.
P5.10 - Nucleolar variant of ErbB3 regulates RNA polymerase I activity XV FISV Sapienza University of Rome 2018 / Ponti, Donatella. - (2018). ( XV FISV Sapienza University of Rome 2018 Roma ).
P5.10 - Nucleolar variant of ErbB3 regulates RNA polymerase I activity XV FISV Sapienza University of Rome 2018
PONTI DONATELLA
2018
Abstract
P5.10 - Nucleolar variant of ErbB3 regulates RNA polymerase I activity Donatella Ponti. Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy The nucleolus is the primary site of the transcription of the ribosomal genes. Rapid growth and division of cells, including tumor cells, is correlated with intensive protein biosynthesis. We have identified a short variant of ErbB3 that localizes in the nucleolus of several cell lines, and, in this compartment it interacts directly with UBF a major transcriptional factor that modulates RNA Pol I activity. Interestingly the presence of this short variant in the nucleolus is inversed correlated with the level of pre-rRNA.The localization of ErbB3 in the cell is under neuregulin control. By performing experiments of immunoprecipitation with anti ErbB3 antibodies, following by immunoblotting we have demonstrated that the interaction between the ErbB3 variant and UBF depends by neuregulin stimulation.ErbB3 receptor represent an emerging cancer target because it plays an important role in drug resistance therapies: interfere with the function of this ErbB3 nucleolar variant could represent an innovative approach to counteract cancer progression.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
