The pressure towards innovation and creation of new model systems in regenerative medicine and cancer research has fostered the development of novel potential therapeutic applications. Kidney injuries provoke a high request of organ transplants making it the most demanding system in the field of regenerative medicine. Furthermore, renal cancer frequently threaten patients’ life and aggressive forms still remain difficult to treat. Ethical issues related to the use of embryonic stem cells, has fueled research on adult, patient-specific pluripotent stem cells as a model for discovery and therapeutic development, but to date, normal and cancerous renal experimental models are lacking. Several research groups are focusing on the development of organoid cultures. Since organoids mimic the original tissue architecture in vitro, they represent an excellent model for tissue engineering studies and cancer therapy testing. We established normal and tumor renal cell carcinoma organoids previously maintained in a heterogeneous multi-clone stem cell-like enriching medium. Starting from adult normal kidney specimens, we were able to isolate and propagate organoid 3D-structures composed of both differentiated and undifferentiated cells while expressing nephron specific markers. Furthermore, we were capable to establish organoids derived from cancer tissues although with a success rate inferior to that of their normal counterpart. Cancer cultures displayed epithelial and mesenchymal phenotype while retaining tumor specific markers. Of note, tumor organoids recapitulated neoplastic masses when orthotopically injected into immunocompromised mice. Our data suggest an innovative approach of long-term establishment of normal- and cancer-derived renal organoids obtained from cultures of fleshly dissociated adult tissues. Our results pave the way to organ replacement pioneering strategies as well as to new models for studying drug-induced nephrotoxicity and renal diseases. Along similar lines, deriving organoids from renal cancer patients opens unprecedented opportunities for generation of preclinical models aimed at improving therapeutic treatments.

Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases / Grassi, L.; Alfonsi, R.; Francescangeli, F.; Signore, M.; De Angelis, M. L.; Addario, A.; Costantini, M.; Flex, E.; Ciolfi, A.; Pizzi, S.; Bruselles, A.; Pallocca, M.; Simone, G.; Haoui, M.; Falchi, M.; Milella, M.; Sentinelli, S.; Di Matteo, P.; Stellacci, E.; Gallucci, M.; Muto, G.; Tartaglia, M.; De Maria, R.; Bonci, D.. - In: CELL DEATH & DISEASE. - ISSN 2041-4889. - 10:3(2019), p. 201. [10.1038/s41419-019-1453-0]

Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases

Francescangeli F.;Flex E.;Pallocca M.;Falchi M.;Gallucci M.;
2019

Abstract

The pressure towards innovation and creation of new model systems in regenerative medicine and cancer research has fostered the development of novel potential therapeutic applications. Kidney injuries provoke a high request of organ transplants making it the most demanding system in the field of regenerative medicine. Furthermore, renal cancer frequently threaten patients’ life and aggressive forms still remain difficult to treat. Ethical issues related to the use of embryonic stem cells, has fueled research on adult, patient-specific pluripotent stem cells as a model for discovery and therapeutic development, but to date, normal and cancerous renal experimental models are lacking. Several research groups are focusing on the development of organoid cultures. Since organoids mimic the original tissue architecture in vitro, they represent an excellent model for tissue engineering studies and cancer therapy testing. We established normal and tumor renal cell carcinoma organoids previously maintained in a heterogeneous multi-clone stem cell-like enriching medium. Starting from adult normal kidney specimens, we were able to isolate and propagate organoid 3D-structures composed of both differentiated and undifferentiated cells while expressing nephron specific markers. Furthermore, we were capable to establish organoids derived from cancer tissues although with a success rate inferior to that of their normal counterpart. Cancer cultures displayed epithelial and mesenchymal phenotype while retaining tumor specific markers. Of note, tumor organoids recapitulated neoplastic masses when orthotopically injected into immunocompromised mice. Our data suggest an innovative approach of long-term establishment of normal- and cancer-derived renal organoids obtained from cultures of fleshly dissociated adult tissues. Our results pave the way to organ replacement pioneering strategies as well as to new models for studying drug-induced nephrotoxicity and renal diseases. Along similar lines, deriving organoids from renal cancer patients opens unprecedented opportunities for generation of preclinical models aimed at improving therapeutic treatments.
2019
humans; kidney; organoids; precision medicine; regenerative medicine; renal insufficiency, chronic
01 Pubblicazione su rivista::01a Articolo in rivista
Organoids as a new model for improving regenerative medicine and cancer personalized therapy in renal diseases / Grassi, L.; Alfonsi, R.; Francescangeli, F.; Signore, M.; De Angelis, M. L.; Addario, A.; Costantini, M.; Flex, E.; Ciolfi, A.; Pizzi, S.; Bruselles, A.; Pallocca, M.; Simone, G.; Haoui, M.; Falchi, M.; Milella, M.; Sentinelli, S.; Di Matteo, P.; Stellacci, E.; Gallucci, M.; Muto, G.; Tartaglia, M.; De Maria, R.; Bonci, D.. - In: CELL DEATH & DISEASE. - ISSN 2041-4889. - 10:3(2019), p. 201. [10.1038/s41419-019-1453-0]
File allegati a questo prodotto
File Dimensione Formato  
Grassi_Organoids-new-model_2019.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 2.77 MB
Formato Adobe PDF
2.77 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1418474
Citazioni
  • ???jsp.display-item.citation.pmc??? 42
  • Scopus 95
  • ???jsp.display-item.citation.isi??? 95
social impact