Guanine-quadruplexes (G4) included in RNA molecules exert several functions in controlling gene expression at post-transcriptional level; however, the molecular mechanisms of G4-mediated regulation are still poorly understood. Here, we describe a regulatory circuitry operating in the early phases of murine muscle differentiation in which a long non-coding RNA (SMaRT) base pairs with a G4-containing mRNA (Mlx-γ) and represses its translation by counteracting the activity of the DHX36 RNA helicase. The time-restricted, specific effect of lnc-SMaRT on the translation of Mlx-γ isoform modulates the general subcellular localization of total MLX proteins, impacting on their transcriptional output and promoting proper myogenesis and mature myotube formation. Therefore, the circuitry made of lnc-SMaRT, Mlx-γ, and DHX36 not only plays an important role in the control of myogenesis but also unravels a molecular mechanism where G4 structures and G4 unwinding activities are regulated in living cells.

SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase / Martone, J.; Mariani, D.; Santini, T.; Setti, A.; Shamloo, S.; Colantoni, A.; Capparelli, F.; Paiardini, A.; Dimartino, D.; Morlando, M.; Bozzoni, I.. - In: EMBO REPORTS. - ISSN 1469-221X. - (2020), p. e49942. [10.15252/embr.201949942]

SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase

Martone J.
Co-primo
;
Mariani D.
Co-primo
;
Santini T.;Setti A.;Shamloo S.;Colantoni A.;Capparelli F.;Paiardini A.;Dimartino D.;Morlando M.;Bozzoni I.
Ultimo
2020

Abstract

Guanine-quadruplexes (G4) included in RNA molecules exert several functions in controlling gene expression at post-transcriptional level; however, the molecular mechanisms of G4-mediated regulation are still poorly understood. Here, we describe a regulatory circuitry operating in the early phases of murine muscle differentiation in which a long non-coding RNA (SMaRT) base pairs with a G4-containing mRNA (Mlx-γ) and represses its translation by counteracting the activity of the DHX36 RNA helicase. The time-restricted, specific effect of lnc-SMaRT on the translation of Mlx-γ isoform modulates the general subcellular localization of total MLX proteins, impacting on their transcriptional output and promoting proper myogenesis and mature myotube formation. Therefore, the circuitry made of lnc-SMaRT, Mlx-γ, and DHX36 not only plays an important role in the control of myogenesis but also unravels a molecular mechanism where G4 structures and G4 unwinding activities are regulated in living cells.
2020
G-quadruplex; lncRNAs; Myogenesis; RNA helicases; RNA–RNA interactions
01 Pubblicazione su rivista::01a Articolo in rivista
SMaRT lncRNA controls translation of a G-quadruplex-containing mRNA antagonizing the DHX36 helicase / Martone, J.; Mariani, D.; Santini, T.; Setti, A.; Shamloo, S.; Colantoni, A.; Capparelli, F.; Paiardini, A.; Dimartino, D.; Morlando, M.; Bozzoni, I.. - In: EMBO REPORTS. - ISSN 1469-221X. - (2020), p. e49942. [10.15252/embr.201949942]
File allegati a questo prodotto
File Dimensione Formato  
Martone_SMaRT_2020.pdf

accesso aperto

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Creative commons
Dimensione 3.29 MB
Formato Adobe PDF
3.29 MB Adobe PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1416609
Citazioni
  • ???jsp.display-item.citation.pmc??? 11
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 17
social impact