Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which are antioxidant and antidiabetic. In the present study, an ethyl acetate extract, obtained by Soxhlet from the peel of Dente di Cavallo DC2 pomegranate (PGE) and characterized to contain 4% w/w of ellagic acid, has been evaluated for its hypoglycemic, antiglycation, and antioxidative cytoprotective properties, in order to provide possible evidence for future nutraceutical applications. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation, and metal chelating abilities were studied. Moreover, the possible antioxidant cytoprotective properties of PGE under hyperglycemic conditions were assayed. Phenolic profile of the extract was characterized by integrated chromatographic and spectrophotometric methods. PGE resulted able to strongly inhibit the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it counteracted the intracellular oxidative stress under hyperglycemic conditions, by reducing the levels of reactive oxygen species and total glutathione. Among the identified phenolics, rutin was the most abundant flavonoid (about 4 % w/w). Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.

Hypoglycemic, antiglycation, and cytoprotective properties of a phenol-rich extract from waste peel of punica granatum L. Var. Dente di cavallo DC2 / Di Sotto, A.; Locatelli, M.; Macone, A.; Toniolo, C.; Cesa, S.; Carradori, S.; Eufemi, M.; Mazzanti, G.; Di Giacomo, S.. - In: MOLECULES. - ISSN 1420-3049. - 24:17(2019), pp. 1-22. [10.3390/molecules24173103]

Hypoglycemic, antiglycation, and cytoprotective properties of a phenol-rich extract from waste peel of punica granatum L. Var. Dente di cavallo DC2

Di Sotto A.
;
Macone A.;Toniolo C.;Cesa S.;Eufemi M.;Mazzanti G.;Di Giacomo S.
2019

Abstract

Pomegranate peel is a natural source of phenolics, claimed to possess healing properties, among which are antioxidant and antidiabetic. In the present study, an ethyl acetate extract, obtained by Soxhlet from the peel of Dente di Cavallo DC2 pomegranate (PGE) and characterized to contain 4% w/w of ellagic acid, has been evaluated for its hypoglycemic, antiglycation, and antioxidative cytoprotective properties, in order to provide possible evidence for future nutraceutical applications. The α-amylase and α-glucosidase enzyme inhibition, interference with advanced glycation end-products (AGE) formation, and metal chelating abilities were studied. Moreover, the possible antioxidant cytoprotective properties of PGE under hyperglycemic conditions were assayed. Phenolic profile of the extract was characterized by integrated chromatographic and spectrophotometric methods. PGE resulted able to strongly inhibit the tested enzymes, especially α-glucosidase, and exerted chelating and antiglycation properties. Also, it counteracted the intracellular oxidative stress under hyperglycemic conditions, by reducing the levels of reactive oxygen species and total glutathione. Among the identified phenolics, rutin was the most abundant flavonoid (about 4 % w/w). Present results suggest PGE to be a possible remedy for hyperglycemia management and encourage further studies to exploit its promising properties.
2019
antioxidant activity; chelating activity; hyperglycemia-induced oxidative stress; Inhibition of advanced glycation end-products; phenolics; vegetable waste; α-amylase; α-glucosidase; antioxidants; cell line; chromatography, high pressure liquid; glutathione; glycoside hydrolase inhibitors; humans; hypoglycemic agents; phenols; plant extracts; pomegranate; reactive oxygen species; Rutin; alpha-Amylases
01 Pubblicazione su rivista::01a Articolo in rivista
Hypoglycemic, antiglycation, and cytoprotective properties of a phenol-rich extract from waste peel of punica granatum L. Var. Dente di cavallo DC2 / Di Sotto, A.; Locatelli, M.; Macone, A.; Toniolo, C.; Cesa, S.; Carradori, S.; Eufemi, M.; Mazzanti, G.; Di Giacomo, S.. - In: MOLECULES. - ISSN 1420-3049. - 24:17(2019), pp. 1-22. [10.3390/molecules24173103]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1416191
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