Adenosine deaminase acting on RNA1 (ADAR1) was previously reported to affect HIV-1 replication. We report data showing that ADAR1 interacts with the HIV-1 p55 Gag protein, the major structural protein of the immature virus capsid. Furthermore, we found that the endogenous ADAR1 is incorporated into virions purified from the supernatant of primary HIV-1-infected CD4+ T lymphocytes. Additional experiments demonstrated that the expression of the p55 Gag protein is sufficient for ADAR1 incorporation into virus-like particles (VLPs).Overall, our data originally support the evidence that ADAR1 can be part of the cell protein array uploaded in HIV-1 particles.
The ADAR1 editing enzyme is encapsidated into HIV-1 virions / Orecchini, E.; Federico, M.; Doria, M.; Arenaccio, C.; Giuliani, E.; Ciafre, S. A.; Michienzi, A.. - In: VIROLOGY. - ISSN 0042-6822. - 485:(2015), pp. 475-480. [10.1016/j.virol.2015.07.027]
The ADAR1 editing enzyme is encapsidated into HIV-1 virions
Orecchini E.;Doria M.;
2015
Abstract
Adenosine deaminase acting on RNA1 (ADAR1) was previously reported to affect HIV-1 replication. We report data showing that ADAR1 interacts with the HIV-1 p55 Gag protein, the major structural protein of the immature virus capsid. Furthermore, we found that the endogenous ADAR1 is incorporated into virions purified from the supernatant of primary HIV-1-infected CD4+ T lymphocytes. Additional experiments demonstrated that the expression of the p55 Gag protein is sufficient for ADAR1 incorporation into virus-like particles (VLPs).Overall, our data originally support the evidence that ADAR1 can be part of the cell protein array uploaded in HIV-1 particles.File | Dimensione | Formato | |
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