We apply mid-infrared absorption microspectroscopy to investigate the light-induced conformational changes of bacteriorhodopsin embedded in native purple membranes deposited on gold substrates. The difference spectra (visible light ON-visible light OFF) obtained on thin films of bacteriorhodopsin display main spectral features connected to the light-induced proton pump process, representing a promising preliminary step towards the investigation of light-sensitive proteins by means of mid-IR tip-enhanced nanospectroscopy.
Light-induced conformational changes of protein receptors probed by difference mid-IR microspectroscopy / Giliberti, V., Polito, R., Ritter, E., Nucara, A., Calvani, P., Broser, M., Hegemann, P., Puskar, L., Schade, U., Baldassarre, L., Ortolani, M.. - 2018-:(2018), pp. 1-2. (43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018 Nagoya Congress Center (NCC), jpn ) [10.1109/IRMMW-THz.2018.8510076].
Light-induced conformational changes of protein receptors probed by difference mid-IR microspectroscopy
Polito R.;Nucara A.;Calvani P.;Baldassarre L.;Ortolani M.
2018
Abstract
We apply mid-infrared absorption microspectroscopy to investigate the light-induced conformational changes of bacteriorhodopsin embedded in native purple membranes deposited on gold substrates. The difference spectra (visible light ON-visible light OFF) obtained on thin films of bacteriorhodopsin display main spectral features connected to the light-induced proton pump process, representing a promising preliminary step towards the investigation of light-sensitive proteins by means of mid-IR tip-enhanced nanospectroscopy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
