We apply mid-infrared absorption microspectroscopy to investigate the light-induced conformational changes of bacteriorhodopsin embedded in native purple membranes deposited on gold substrates. The difference spectra (visible light ON-visible light OFF) obtained on thin films of bacteriorhodopsin display main spectral features connected to the light-induced proton pump process, representing a promising preliminary step towards the investigation of light-sensitive proteins by means of mid-IR tip-enhanced nanospectroscopy.
Light-induced conformational changes of protein receptors probed by difference mid-IR microspectroscopy / Giliberti, V.; Polito, R.; Ritter, E.; Nucara, A.; Calvani, P.; Broser, M.; Hegemann, P.; Puskar, L.; Schade, U.; Baldassarre, L.; Ortolani, M.. - 2018-:(2018), pp. 1-2. ((Intervento presentato al convegno 43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018 tenutosi a Nagoya Congress Center (NCC), jpn [10.1109/IRMMW-THz.2018.8510076].