We apply mid-infrared absorption microspectroscopy to investigate the light-induced conformational changes of bacteriorhodopsin embedded in native purple membranes deposited on gold substrates. The difference spectra (visible light ON-visible light OFF) obtained on thin films of bacteriorhodopsin display main spectral features connected to the light-induced proton pump process, representing a promising preliminary step towards the investigation of light-sensitive proteins by means of mid-IR tip-enhanced nanospectroscopy.

Light-induced conformational changes of protein receptors probed by difference mid-IR microspectroscopy / Giliberti, V.; Polito, R.; Ritter, E.; Nucara, A.; Calvani, P.; Broser, M.; Hegemann, P.; Puskar, L.; Schade, U.; Baldassarre, L.; Ortolani, M.. - 2018-:(2018), pp. 1-2. ((Intervento presentato al convegno 43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018 tenutosi a Nagoya Congress Center (NCC), jpn [10.1109/IRMMW-THz.2018.8510076].

Light-induced conformational changes of protein receptors probed by difference mid-IR microspectroscopy

Polito R.;Nucara A.;Calvani P.;Baldassarre L.;Ortolani M.
2018

Abstract

We apply mid-infrared absorption microspectroscopy to investigate the light-induced conformational changes of bacteriorhodopsin embedded in native purple membranes deposited on gold substrates. The difference spectra (visible light ON-visible light OFF) obtained on thin films of bacteriorhodopsin display main spectral features connected to the light-induced proton pump process, representing a promising preliminary step towards the investigation of light-sensitive proteins by means of mid-IR tip-enhanced nanospectroscopy.
43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018
04 Pubblicazione in atti di convegno::04d Abstract in atti di convegno
Light-induced conformational changes of protein receptors probed by difference mid-IR microspectroscopy / Giliberti, V.; Polito, R.; Ritter, E.; Nucara, A.; Calvani, P.; Broser, M.; Hegemann, P.; Puskar, L.; Schade, U.; Baldassarre, L.; Ortolani, M.. - 2018-:(2018), pp. 1-2. ((Intervento presentato al convegno 43rd International Conference on Infrared Millimeter and Terahertz Waves, IRMMW-THz 2018 tenutosi a Nagoya Congress Center (NCC), jpn [10.1109/IRMMW-THz.2018.8510076].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1410267
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