Statement of Purpose: Human adipose-derived stem cells (hASCs) express vascular endothelial growth factor (VEGF) as one of the regulators of their proliferation and differentiation. To enhance the expression of this growth factor in hASCs, non-viral transfection methods such as cationic polymers, lipids, and peptide conjugates are attractive candidates. Aside from the type of carrier, internalization of plasmid DNA encoded for VEGF is also dependent on the elasticity of the cell’s substrate which controls the cytoskeletal remodeling. This study investigates the role of substrate stiffness on non-viral transfection of hASCs with the aim to maximize hASCs expression of VEGF.
The role of substrate stiffness in stem cell transfection of plasmid DNA using lipid-based nanocarriers / Modaresi, S.; Pacelli, S.; Whitlow, J.; Paul, A.. - 40:(2019), p. 361. (Intervento presentato al convegno 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence tenutosi a Seattle, United States).
The role of substrate stiffness in stem cell transfection of plasmid DNA using lipid-based nanocarriers
Pacelli S.;
2019
Abstract
Statement of Purpose: Human adipose-derived stem cells (hASCs) express vascular endothelial growth factor (VEGF) as one of the regulators of their proliferation and differentiation. To enhance the expression of this growth factor in hASCs, non-viral transfection methods such as cationic polymers, lipids, and peptide conjugates are attractive candidates. Aside from the type of carrier, internalization of plasmid DNA encoded for VEGF is also dependent on the elasticity of the cell’s substrate which controls the cytoskeletal remodeling. This study investigates the role of substrate stiffness on non-viral transfection of hASCs with the aim to maximize hASCs expression of VEGF.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
