Plasmodium parasites infect non-human primates (NHP) in tropical regions around the world. In Latin America, NHP are potential reservoirs of Plasmodium brasilianum/Plasmodium malariae, and some NHP species have been found infected with Plasmodium falciparum and Plasmodium simium, as reported previously. Considering the risk of infection to humans associated with the presence of NHP and Anopheles infected with 115 Plasmodium spp., this study aimed to determine the circulating species of Plasmodium in vectors and NHP species living in fragmented forest areas in Colombia. Sampling was conducted in five different forest fragments. Primates were followed and faecal samples were collected immediately after defecation, and placed in tubes with RNAlater solution. Samples from 75 Ateles hybridus, 25 Cebus versicolor, 58 Alouatta seniculus and eight Aotus griseimembra were collected. Blood samples from four A.hybridus, nine C. versicolor, five A. seniculus and seven A. griseimembra were obtained. Adult mosquitoes were sorted after capture and most female Anopheles were preserved in RNAlater buffer, the remaining were kept dry for taxonomic identification. Plasmodium sp. detection was performed through PCR, and second reactions were conducted for Plasmodium falciparum, Plasmodium malariae and Plasmodium vivax detection, using ribosomal and mitocondrial primers. PCR products were visualized on agarose gel and positive samples were sequenced. Mosquito identification was confirmed through DNA barcoding. From faecal samples it was found P.falciparum infecting A.seniculus, while P. malariae infected A. seniculus, A. hybridus and A. griseimembra. P. vivax infected A. hybridus, A. seniculus and C. versicolor. From blood samples it was found P. malariae infecting all the four NHP species tested and P.vivax infected A. hybridus. Infection with P.vivax was confirmed in three Anopheles species and one was positive for P. malariae. This scenario suggests important epidemiological implications in the human - NHP interface and the associated risk of Malaria transmission.
Detection of Plasmodium sp. in non-human primates and mosquitoes in forest fragments in Colombia / Silvia, Rondón; Cielo, León; Andrés, Link; Camila, González. - 99:(2018). (Intervento presentato al convegno American Society of Tropical Medicine & Hygiene - 67th Annual Meeting tenutosi a New Orleans, USA).
Detection of Plasmodium sp. in non-human primates and mosquitoes in forest fragments in Colombia
Silvia Rondón
Primo
;
2018
Abstract
Plasmodium parasites infect non-human primates (NHP) in tropical regions around the world. In Latin America, NHP are potential reservoirs of Plasmodium brasilianum/Plasmodium malariae, and some NHP species have been found infected with Plasmodium falciparum and Plasmodium simium, as reported previously. Considering the risk of infection to humans associated with the presence of NHP and Anopheles infected with 115 Plasmodium spp., this study aimed to determine the circulating species of Plasmodium in vectors and NHP species living in fragmented forest areas in Colombia. Sampling was conducted in five different forest fragments. Primates were followed and faecal samples were collected immediately after defecation, and placed in tubes with RNAlater solution. Samples from 75 Ateles hybridus, 25 Cebus versicolor, 58 Alouatta seniculus and eight Aotus griseimembra were collected. Blood samples from four A.hybridus, nine C. versicolor, five A. seniculus and seven A. griseimembra were obtained. Adult mosquitoes were sorted after capture and most female Anopheles were preserved in RNAlater buffer, the remaining were kept dry for taxonomic identification. Plasmodium sp. detection was performed through PCR, and second reactions were conducted for Plasmodium falciparum, Plasmodium malariae and Plasmodium vivax detection, using ribosomal and mitocondrial primers. PCR products were visualized on agarose gel and positive samples were sequenced. Mosquito identification was confirmed through DNA barcoding. From faecal samples it was found P.falciparum infecting A.seniculus, while P. malariae infected A. seniculus, A. hybridus and A. griseimembra. P. vivax infected A. hybridus, A. seniculus and C. versicolor. From blood samples it was found P. malariae infecting all the four NHP species tested and P.vivax infected A. hybridus. Infection with P.vivax was confirmed in three Anopheles species and one was positive for P. malariae. This scenario suggests important epidemiological implications in the human - NHP interface and the associated risk of Malaria transmission.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
