Three columns packed with 2.0 μm superficially porous particles, 1.7 μm fully porous particles, and monodisperse 1.9 μm fully porous particles with narrow particle size distribution have been deeply characterized from a kinetic point of view. The 1.9 μm column showed excellent kinetic performance, comparable to that of the superficially porous one. These two columns also exhibit flatter c-branches of the van Deemter curve compared to the 1.7 μm fully porous particles column, resulting in smaller loss of efficiency when they are operated at higher flow rates than the optimal ones. The independent evaluation of each contribution to band broadening has revealed that the difference in kinetic performance comes from the very small eddy dispersion contribution on the 1.9 μm column, surprisingly even lower than that of the superficially porous one. This finding suggests a very good packing of the monodisperse 1.9 μm column. On the other hand, the potential of 1.7 μm fully porous particles is completely broken down by the strong frictional heating effect already arising at relatively low flow rates.

Investigation of mass transfer properties and kinetic performance of high-efficiency columns packed with C18 sub-2 μm fully and superficially porous particles / Felletti, S.; De Luca, C.; Lievore, G.; Pasti, L.; Chenet, T.; Mazzoccanti, G.; Gasparrini, F.; Cavazzini, A.; Catani, M.. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9306. - 43:9-10(2020), pp. 1737-1745. [10.1002/jssc.202000041]

Investigation of mass transfer properties and kinetic performance of high-efficiency columns packed with C18 sub-2 μm fully and superficially porous particles

Mazzoccanti G.;Gasparrini F.;
2020

Abstract

Three columns packed with 2.0 μm superficially porous particles, 1.7 μm fully porous particles, and monodisperse 1.9 μm fully porous particles with narrow particle size distribution have been deeply characterized from a kinetic point of view. The 1.9 μm column showed excellent kinetic performance, comparable to that of the superficially porous one. These two columns also exhibit flatter c-branches of the van Deemter curve compared to the 1.7 μm fully porous particles column, resulting in smaller loss of efficiency when they are operated at higher flow rates than the optimal ones. The independent evaluation of each contribution to band broadening has revealed that the difference in kinetic performance comes from the very small eddy dispersion contribution on the 1.9 μm column, surprisingly even lower than that of the superficially porous one. This finding suggests a very good packing of the monodisperse 1.9 μm column. On the other hand, the potential of 1.7 μm fully porous particles is completely broken down by the strong frictional heating effect already arising at relatively low flow rates.
2020
fully porous particles; kinetic performance; octadecyl stationary phase; superficially porous particles; ultra-high-performance liquid chromatography
01 Pubblicazione su rivista::01a Articolo in rivista
Investigation of mass transfer properties and kinetic performance of high-efficiency columns packed with C18 sub-2 μm fully and superficially porous particles / Felletti, S.; De Luca, C.; Lievore, G.; Pasti, L.; Chenet, T.; Mazzoccanti, G.; Gasparrini, F.; Cavazzini, A.; Catani, M.. - In: JOURNAL OF SEPARATION SCIENCE. - ISSN 1615-9306. - 43:9-10(2020), pp. 1737-1745. [10.1002/jssc.202000041]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1391669
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