Mammalian acetylcholinesterase (AChE) gene expression is exquisitely regulated in target tissues and cells during differentiation. An intron located between the first and second exons governs a similar to 100-fold increase in AChE expression during myoblast to myotube differentiation in C2C12 cells. Regulation is confined to 255 bp of evolutionarily conserved sequence containing functional transcription factor consensus motifs that indirectly interact with the endogenous promoter. To examine control in vivo, this region was deleted by homologous recombination. The knock-out mouse is virtually devoid of AChE activity and its encoding mRNA in skeletal muscle, yet activities in brain and spinal cord innervating skeletal muscle are unaltered. The transcription factors MyoD and myocyte enhancer factor-2 appear to be responsible for muscle regulation. Selective control of AChE expression by this region is also found in hematopoietic lineages. Expression patterns in muscle and CNS neurons establish that virtually all AChE activity at the mammalian neuromuscular junction arises from skeletal muscle rather than from biosynthesis in the motoneuron cell body and axoplasmic transport.

Acetylcholinesterase expression in muscle is specifically controlled by a promoter-selective enhancesome in the first intron / S., Camp; DE JACO, Antonella; L., Zhang; M., Marquez; B., De La Torre; P., Taylor. - In: THE JOURNAL OF NEUROSCIENCE. - ISSN 0270-6474. - 28:10(2008), pp. 2459-2470. [10.1523/jneurosci.4600-07.2008]

Acetylcholinesterase expression in muscle is specifically controlled by a promoter-selective enhancesome in the first intron

DE JACO, Antonella;
2008

Abstract

Mammalian acetylcholinesterase (AChE) gene expression is exquisitely regulated in target tissues and cells during differentiation. An intron located between the first and second exons governs a similar to 100-fold increase in AChE expression during myoblast to myotube differentiation in C2C12 cells. Regulation is confined to 255 bp of evolutionarily conserved sequence containing functional transcription factor consensus motifs that indirectly interact with the endogenous promoter. To examine control in vivo, this region was deleted by homologous recombination. The knock-out mouse is virtually devoid of AChE activity and its encoding mRNA in skeletal muscle, yet activities in brain and spinal cord innervating skeletal muscle are unaltered. The transcription factors MyoD and myocyte enhancer factor-2 appear to be responsible for muscle regulation. Selective control of AChE expression by this region is also found in hematopoietic lineages. Expression patterns in muscle and CNS neurons establish that virtually all AChE activity at the mammalian neuromuscular junction arises from skeletal muscle rather than from biosynthesis in the motoneuron cell body and axoplasmic transport.
2008
acetylcholinesterase; differentiation; enhancesome; knock-out mouse; muscle; myogenesis
01 Pubblicazione su rivista::01a Articolo in rivista
Acetylcholinesterase expression in muscle is specifically controlled by a promoter-selective enhancesome in the first intron / S., Camp; DE JACO, Antonella; L., Zhang; M., Marquez; B., De La Torre; P., Taylor. - In: THE JOURNAL OF NEUROSCIENCE. - ISSN 0270-6474. - 28:10(2008), pp. 2459-2470. [10.1523/jneurosci.4600-07.2008]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/138660
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 26
  • ???jsp.display-item.citation.isi??? 26
social impact