The Dorsal Raphe (DR) is the primary source of serotonergic input in the brain and a center for the homeostatic maintenance of the serotonergic tone. Under repeated stimulation, it can undergo adaptive modifications that alter serotonergic neurotransmission, which can lead to behavioral dysfunction. Post-transcriptional regulation by microRNAs is implicated in these adaptations. However, a global microRNA/target network effect on the DR neuroplasticity has yet to be elucidated. Here we investigate the microRNAs/mRNAs regulatory activity in the mouse DR after a chronic stress experience. First, we assessed the behavioral consequences of repeated restraint stress exposure and the functional adaptations of the DR by measuring the change in acute stress-induced serotonin release. Then, through next generation RNA-Seq of Argonaute2-bound RNA (RISC-Seq) we identified microRNAs and their targets that are associated to the RISC complex of the DR in unstressed and stressed mice. We mapped the potential microRNA/mRNA network within the stress-altered transcripts, uncovering new interactions that contribute to the chronic stress-induced DR modifications.

RISC RNA sequencing in the Dorsal Raphè reveals microRNAs regulatory activities associated with behavioral and functional adaptations to chronic stress / Babicola, L; Pietrosanto, M; Ielpo, D; D'Addario, Sl; Cabib, S; Ventura, R; Ferlazzo, F; Helmer-Citterich, M; Andolina, D; Lo Iacono, L.. - In: BRAIN RESEARCH. - ISSN 0006-8993. - 1736:(2020). [10.1016/j.brainres.2020.146763]

RISC RNA sequencing in the Dorsal Raphè reveals microRNAs regulatory activities associated with behavioral and functional adaptations to chronic stress

Babicola L;Ielpo D;D'Addario SL;Cabib S;Ventura R;Ferlazzo F;Andolina D;Lo Iacono L.
2020

Abstract

The Dorsal Raphe (DR) is the primary source of serotonergic input in the brain and a center for the homeostatic maintenance of the serotonergic tone. Under repeated stimulation, it can undergo adaptive modifications that alter serotonergic neurotransmission, which can lead to behavioral dysfunction. Post-transcriptional regulation by microRNAs is implicated in these adaptations. However, a global microRNA/target network effect on the DR neuroplasticity has yet to be elucidated. Here we investigate the microRNAs/mRNAs regulatory activity in the mouse DR after a chronic stress experience. First, we assessed the behavioral consequences of repeated restraint stress exposure and the functional adaptations of the DR by measuring the change in acute stress-induced serotonin release. Then, through next generation RNA-Seq of Argonaute2-bound RNA (RISC-Seq) we identified microRNAs and their targets that are associated to the RISC complex of the DR in unstressed and stressed mice. We mapped the potential microRNA/mRNA network within the stress-altered transcripts, uncovering new interactions that contribute to the chronic stress-induced DR modifications.
2020
Chronic stress; dorsal raphe; microRNA regulatory activity; RISC sequencing; research domain criteria
01 Pubblicazione su rivista::01a Articolo in rivista
RISC RNA sequencing in the Dorsal Raphè reveals microRNAs regulatory activities associated with behavioral and functional adaptations to chronic stress / Babicola, L; Pietrosanto, M; Ielpo, D; D'Addario, Sl; Cabib, S; Ventura, R; Ferlazzo, F; Helmer-Citterich, M; Andolina, D; Lo Iacono, L.. - In: BRAIN RESEARCH. - ISSN 0006-8993. - 1736:(2020). [10.1016/j.brainres.2020.146763]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1385624
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