During a survey of the mutations of the low density lipoprotein receptor (LDL-R) gene in Italian patients with familial hypercholesterolemia (FH), we identified a novel point mutation, that creates a new EcoRI site at the 5′ end of exon 7, in a heterozygous FH subject (FH-100). The sequence of a cDNA fragment encompassing exon 7 showed the presence of a G→T transversion in codon 297; this created a new EcoRI site and produced a missense mutation, leading to a Cys297→Phe substitution in repeat A of the epidermal growth factor (EGF) precursor homology domain of LDL-R. Since the substitution of Cys297 disrupts the intracellular transport of the LDL-R protein, as previously demonstrated by site-directed mutagenesis, we suggest that this mutation is the cause of FH in the FH-100 proband. We screened the DNA of 303 Italian FH patients by amplification of exon 7 from genomic DNA followed by digestion with EcoRI or by Southern blotting. Two individuals (FH-64 and FH-127) were found to be carriers of the Cys297→Phe mutation. Restriction fragment length polymorphism analysis demonstrated that, in two kindreds (FH-64 and FH-100), the haplotype in linkage with the Cys297→Phe mutation was the same, suggesting the presence of a common ancestor. The Cys297→Phe mutation has been designated FHTrieste after the name of the city in Northern Italy from which probands FH-100 and FH-127 originate.

A new missense mutation (Cys 297- - Phe) of the low density lipoprotein receptor in italian patients with familial hypercholesterolemia (FH Trieste) / Lelli, N; Garuti, R; Pedrazzi, P; Ghisellini, M; Simone, Ml; Tiozzo, R; Cattin, L; Valenti, M; Rolleri, M; Bertolini, S; Stefanutti, Claudia; Calandra, S.. - In: HUMAN GENETICS. - ISSN 0340-6717. - STAMPA. - 93:(1994), pp. 538-540. [10.1007/BF00202819]

A new missense mutation (Cys 297- - Phe) of the low density lipoprotein receptor in italian patients with familial hypercholesterolemia (FH Trieste)

STEFANUTTI, Claudia;
1994

Abstract

During a survey of the mutations of the low density lipoprotein receptor (LDL-R) gene in Italian patients with familial hypercholesterolemia (FH), we identified a novel point mutation, that creates a new EcoRI site at the 5′ end of exon 7, in a heterozygous FH subject (FH-100). The sequence of a cDNA fragment encompassing exon 7 showed the presence of a G→T transversion in codon 297; this created a new EcoRI site and produced a missense mutation, leading to a Cys297→Phe substitution in repeat A of the epidermal growth factor (EGF) precursor homology domain of LDL-R. Since the substitution of Cys297 disrupts the intracellular transport of the LDL-R protein, as previously demonstrated by site-directed mutagenesis, we suggest that this mutation is the cause of FH in the FH-100 proband. We screened the DNA of 303 Italian FH patients by amplification of exon 7 from genomic DNA followed by digestion with EcoRI or by Southern blotting. Two individuals (FH-64 and FH-127) were found to be carriers of the Cys297→Phe mutation. Restriction fragment length polymorphism analysis demonstrated that, in two kindreds (FH-64 and FH-100), the haplotype in linkage with the Cys297→Phe mutation was the same, suggesting the presence of a common ancestor. The Cys297→Phe mutation has been designated FHTrieste after the name of the city in Northern Italy from which probands FH-100 and FH-127 originate.
1994
01 Pubblicazione su rivista::01a Articolo in rivista
A new missense mutation (Cys 297- - Phe) of the low density lipoprotein receptor in italian patients with familial hypercholesterolemia (FH Trieste) / Lelli, N; Garuti, R; Pedrazzi, P; Ghisellini, M; Simone, Ml; Tiozzo, R; Cattin, L; Valenti, M; Rolleri, M; Bertolini, S; Stefanutti, Claudia; Calandra, S.. - In: HUMAN GENETICS. - ISSN 0340-6717. - STAMPA. - 93:(1994), pp. 538-540. [10.1007/BF00202819]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/137644
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