In this study we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection to one colonized patient. In the multi-species cluster the bla VIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the bla SHV-12, qnrS1 and mph(A) genes, located on a highly conjugative and broad-host range IncA plasmid. Characteristics and origin of this IncA plasmid were studied.

A multi-species bunch of VIM-1 Carbapenemase producing enterobacterales linked by a novel, highly conjugative and broad-host range IncA plasmid, menaces the re-emergence of VIM-1 / Arcari, G; Di Lella, Fm; Bibbolino, G; Mengoni, F; Beccaccioli, M; Antonelli, G; Faino, L; Carattoli, A. - In: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. - ISSN 1098-6596. - (2020), pp. 1-27. [10.1128/AAC.02435-19]

A multi-species bunch of VIM-1 Carbapenemase producing enterobacterales linked by a novel, highly conjugative and broad-host range IncA plasmid, menaces the re-emergence of VIM-1

G Arcari;F Mengoni;M Beccaccioli;G Antonelli;L Faino;A Carattoli
2020

Abstract

In this study we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection to one colonized patient. In the multi-species cluster the bla VIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the bla SHV-12, qnrS1 and mph(A) genes, located on a highly conjugative and broad-host range IncA plasmid. Characteristics and origin of this IncA plasmid were studied.
File allegati a questo prodotto
File Dimensione Formato  
Arcari_A-multi-species_2020.pdf

solo gestori archivio

Tipologia: Documento in Post-print (versione successiva alla peer review e accettata per la pubblicazione)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 1.89 MB
Formato Adobe PDF
1.89 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1367755
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 8
social impact