The CellSearch® system which is still considered the gold standard for the enumeration of circulating tumor cells (CTC) utilizes antibodies against the epithelial cell adhesion molecule (EpCAM) for CTC enrichment. Recently, CTC discarded by the CellSearch® system due to their low EpCAM expression have been isolated and analyzed. We here sought to discuss technical and biological issues concerning the isolation and characterization of EpCAMlow CTC, highlighting the enormous potential of this subpopulation discarded by CellSearch®, which might instead reveal an unexpected clinical significance in tumor types where CTC enumeration has never been validated for prognostic and predictive purpose.

EpCAMlow circulating tumor cells: Gold in the waste / Nicolazzo, C.; Gradilone, A.; Loreni, F.; Raimondi, C.; Gazzaniga, P.. - In: DISEASE MARKERS. - ISSN 0278-0240. - 2019:(2019). [10.1155/2019/1718920]

EpCAMlow circulating tumor cells: Gold in the waste

Nicolazzo C.;Gradilone A.;Loreni F.;Gazzaniga P.
2019

Abstract

The CellSearch® system which is still considered the gold standard for the enumeration of circulating tumor cells (CTC) utilizes antibodies against the epithelial cell adhesion molecule (EpCAM) for CTC enrichment. Recently, CTC discarded by the CellSearch® system due to their low EpCAM expression have been isolated and analyzed. We here sought to discuss technical and biological issues concerning the isolation and characterization of EpCAMlow CTC, highlighting the enormous potential of this subpopulation discarded by CellSearch®, which might instead reveal an unexpected clinical significance in tumor types where CTC enumeration has never been validated for prognostic and predictive purpose.
2019
epcam, circulating tumor cells
01 Pubblicazione su rivista::01a Articolo in rivista
EpCAMlow circulating tumor cells: Gold in the waste / Nicolazzo, C.; Gradilone, A.; Loreni, F.; Raimondi, C.; Gazzaniga, P.. - In: DISEASE MARKERS. - ISSN 0278-0240. - 2019:(2019). [10.1155/2019/1718920]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1345814
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