Fabrication of a novel biocatalyst with Laccase immobilized on eggshell membrane

The research provides the suitability of a not-lignocellulosic agro-industrial wastes, such as egg shell membrane, for laccase from Trametes versicolor immobilization. This result is of significant interest due to the low cost and great availability of such waste, which actually is underused and causes significant environmental problem for improper storage. In addition, the development of economic biocatalysts more sustainable, besides reduce environmental impacts, improve the application of enzymatic technology in industry. Laccase is a eco-friendly oxidoreductase enzyme and has been used in different industrial fields for different purposes, from food additive and beverage processing to biomedical diagnosis and production of biofuels. It catalyzes the oxidation of a great number of substrates which can be widened to higher‐redox potential compounds than laccase one with the help of laccase-mediator-system . A very simple method was based on the cross-linking reaction between the amino functional groups of the protein and of the ESM solid surface. So, glutaraldehyde, as cross linker, was dropped onto the ESM surface in order to form the Shiff bases .The performance of biocatalyst was evaluated taking into account the operational stability expressed as reuse and the enzyme recovery. The goal of this research is to apply the biocatalyst in the removal of contaminants from waste water.