A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV–visible microspectroscopy in crystallo, solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15–40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O2 in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.

Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments / Ardiccioni, Chiara; Arcovito, Alessandro; Della Longa, Stefano; van der Linden, Peter; Bourgeois, Dominique; Weik, Martin; Montemiglio, LINDA CELESTE; Savino, Carmelinda; Avella, Giovanna; Exertier, Cécile; Carpentier, Philppe; Prangé, Thierry; Brunori, Maurizio; Colloc'H, Nathalie; Vallone, Beatrice. - In: IUCRJ. - ISSN 2052-2525. - 6:5(2019), pp. 832-842. [10.1107/S2052252519008157]

Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments

Ardiccioni Chiara;Arcovito Alessandro;Montemiglio Linda Celeste;Savino Carmelinda;Avella Giovanna;Exertier Cécile;Brunori Maurizio;Vallone Beatrice
2019

Abstract

A combined biophysical approach was applied to map gas-docking sites within murine neuroglobin (Ngb), revealing snapshots of events that might govern activity and dynamics in this unique hexacoordinate globin, which is most likely to be involved in gas-sensing in the central nervous system and for which a precise mechanism of action remains to be elucidated. The application of UV–visible microspectroscopy in crystallo, solution X-ray absorption near-edge spectroscopy and X-ray diffraction experiments at 15–40 K provided the structural characterization of an Ngb photolytic intermediate by cryo-trapping and allowed direct observation of the relocation of carbon monoxide within the distal heme pocket after photodissociation. Moreover, X-ray diffraction at 100 K under a high pressure of dioxygen, a physiological ligand of Ngb, unravelled the existence of a storage site for O2 in Ngb which coincides with Xe-III, a previously described docking site for xenon or krypton. Notably, no other secondary sites were observed under our experimental conditions.
2019
CO photolysis; XANES; cryo-trapping; crystal microspectroscopy; heme protein; neuroglobin; neuroprotection; oxygen binding; protein structure; soak-and-freeze pressurization; structural biology; structure determination; ultralow-temperature X-ray crystallography
01 Pubblicazione su rivista::01a Articolo in rivista
Ligand pathways in neuroglobin revealed by low-temperature photodissociation and docking experiments / Ardiccioni, Chiara; Arcovito, Alessandro; Della Longa, Stefano; van der Linden, Peter; Bourgeois, Dominique; Weik, Martin; Montemiglio, LINDA CELESTE; Savino, Carmelinda; Avella, Giovanna; Exertier, Cécile; Carpentier, Philppe; Prangé, Thierry; Brunori, Maurizio; Colloc'H, Nathalie; Vallone, Beatrice. - In: IUCRJ. - ISSN 2052-2525. - 6:5(2019), pp. 832-842. [10.1107/S2052252519008157]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1335877
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